Human CD14 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB3833
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
ELISA Capture (Matched Antibody Pair)
Cited:
Binding Assay, ELISA Capture, ELISA Microarray Development
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 50040
Product Specifications
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human CD14
Thr20-Cys352
Accession # P08571
Thr20-Cys352
Accession # P08571
Specificity
Detects human CD14 in sandwich ELISAs.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human CD14 Antibody
Human CD14 ELISA Standard Curve
Recombinant Human CD14 (Catalog # 383-CD) was serially diluted and captured by Mouse Anti-Human CD14 Monoclonal Antibody (Catalog # MAB3833) coated on a Clear Polystyrene Microplate (Catalog # DY990). Detection antibody was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)Applications for Human CD14 Antibody
Human CD14 Sandwich Immunoassay
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Reviewed Applications
Read 2 reviews rated 4.5 using MAB3833 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS and NaCl with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CD14
CD14 occurs both as a secreted protein and as a GPI-linked membrane protein. It plays a critical role in lipopolysaccharide (LPS)-dependent signal transduction.
Alternate Names
CD14
Gene Symbol
CD14
UniProt
Additional CD14 Products
Product Documents for Human CD14 Antibody
Product Specific Notices for Human CD14 Antibody
For research use only
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