Human CCL5/RANTES Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB278
Scientific images may be resized, cropped, or adjusted for brightness or contrast for presentation purposes. These updates are limited to presentation and do not affect the underlying scientific interpretation of the data. Where available, additional source data may be provided upon request.
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Immunohistochemistry, Western Blot, Neutralization, Intracellular Staining by Flow Cytometry, CyTOF-ready
Cited:
Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Neutralization, Flow Cytometry, Immunocytochemistry, ELISA Development
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 21445
Product Specifications
Immunogen
E. coli-derived recombinant human CCL5/RANTES
Specificity
Detects human CCL5/RANTES in direct ELISAs and Western blots. In direct ELISAs and Western blots, this antibody does not cross-react with other chemokines, including recombinant human CCL2, 3, 4, recombinant mouse (rm) CCL3, and rmCCL4.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Scientific Data Images for Human CCL5/RANTES Antibody
Chemotaxis Induced by CCL5/RANTES and Neutralization by Human CCL5/RANTES Antibody.
Recombinant Human CCL5/RANTES (Catalog # 278-RN) chemoattracts the BaF3 mouse pro-B cell line transfected with human CCR5 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CCL5/RANTES (0.01 µg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human CCL5/RANTES Monoclonal Antibody (Catalog # MAB278). The ND50 is typically 0.1-0.4 µg/mL.
CCL5/RANTES in Mouse Small Intestine.
CCL5/RANTES was detected in perfusion fixed frozen sections of mouse small intestine using Mouse Anti-Human CCL5/RANTES Monoclonal Antibody (Catalog # MAB278) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to cytoplasm. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.Applications for Human CCL5/RANTES Antibody
Application
Recommended Usage
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Immunohistochemistry
8-25 µg/mL
Sample: Perfusion fixed frozen sections of mouse small intestine
Sample: Perfusion fixed frozen sections of mouse small intestine
Intracellular Staining by Flow Cytometry
2.5 µg/106 cells
Sample: Human CCL5/RANTES transfected NS0 cells fixed with paraformaldehyde and permeabilized with saponin
Sample: Human CCL5/RANTES transfected NS0 cells fixed with paraformaldehyde and permeabilized with saponin
Western Blot
1 µg/mL
Sample: Recombinant Human CCL5/RANTES (Catalog # 278-RN)
Sample: Recombinant Human CCL5/RANTES (Catalog # 278-RN)
Neutralization
Measured by its ability to neutralize CCL5/RANTES-induced chemotaxis in the BaF3 mouse pro-B cell line transfected with human CCR5. The Neutralization Dose (ND50) is typically 0.1-0.4 µg/mL in the presence of 0.01 µg/mL Recombinant Human CCL5/RANTES.
Reviewed Applications
Read 2 reviews rated 4.5 using MAB278 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from ascites
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CCL5/RANTES
Alternate Names
RANTES, SISd
Gene Symbol
CCL5
Additional CCL5/RANTES Products
Product Documents for Human CCL5/RANTES Antibody
Product Specific Notices for Human CCL5/RANTES Antibody
For research use only
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