Human CCL28 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB7171
Scientific images may be resized, cropped, or adjusted for brightness or contrast for presentation purposes. These updates are limited to presentation and do not affect the underlying scientific interpretation of the data. Where available, additional source data may be provided upon request.
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Western Blot, ELISA Capture (Matched Antibody Pair)
Cited:
Immunohistochemistry, Neutralization
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 62714
Product Specifications
Immunogen
E. coli-derived recombinant human CCL28
Ile23-Tyr127
Accession # Q9NRJ3
Ile23-Tyr127
Accession # Q9NRJ3
Specificity
Detects human CCL28 in ELISAs and Western blots. In sandwich immunoassays, no cross-reactivity or interference with recombinant human CCL27 or recombinant mouse CCL28 is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human CCL28 Antibody
Human CCL28 ELISA Standard Curve
Recombinant Human CCL28 (Catalog # 717-VC) was serially diluted and captured by Mouse Anti-Human CCL28 Monoclonal Antibody (Catalog # MAB7171) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human CCL28 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF717) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)Applications for Human CCL28 Antibody
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: Recombinant Human CCL28 (Catalog # 717-VC)
Sample: Recombinant Human CCL28 (Catalog # 717-VC)
Human CCL28 Sandwich Immunoassay
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CCL28
Human CCL28 (CC chemokine ligand 28) is a member of the CC chemokine superfamily. Human CCL28 cDNA encodes a 127 amino acid (aa) residue precursor protein with a putative 22 aa residue signal peptide that is cleaved to produce the 105 aa residue mature protein. Human and mouse CCL28 share 83% aa identity in their mature regions. Among CC chemokines, CCL28 shares the most homology with CCL27/CTACK. Human and mouse CCL28 RNA is most highly expressed by epithelial cells in normal and pathologic colon. Human CCL28 RNA is also present in normal and asthmatic lung tissues. The receptor for CCL28 is the CCR10 (formerly GPR2 orphan receptor) which is also the receptor for CCL27/CTACK.
References
- Wang, W. et al. (2000) J. Biol. Chem. 275:22313.
Alternate Names
CCL28, MEC, VIC
Gene Symbol
CCL28
UniProt
Additional CCL28 Products
Product Documents for Human CCL28 Antibody
Product Specific Notices for Human CCL28 Antibody
For research use only
Loading...
Loading...
Loading...
Loading...
Loading...