Human CCL18/PARC Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB394

Catalog #
Availability
Size / Price
Qty
MAB394-100
MAB394-500
MAB394-SP
Print Quote
Bulk Order
100% Guarantee

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse

Applications

Validated:

Western Blot, ELISA Capture (Matched Antibody Pair), Intracellular Staining by Flow Cytometry, CyTOF-ready

Cited:

Immunohistochemistry, Immunocytochemistry, ELISA Capture, ELISA Development (Capture), Functional Assay, Luminex Development

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 64507

View all CCL18/PARC Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human CCL18/PARC
Ala21-Ala89
Accession # P55774.1

Specificity

Detects human CCL18/PARC in ELISAs and Western blots. In ELISAs, does not cross-react with recombinant human CCL3, 4, 14, 15, 23, recombinant mouse (rm) CCL3, rmCCL4, recombinant viral (rv) MIP-I or rvMIP‑II.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human CCL18/PARC Antibody

Human CCL18 / PARC ELISA Standard Curve

Human CCL18 / PARC ELISA Standard Curve

Recombinant Human CCL18/PARC (Catalog # 394-PA) was serially diluted and captured by Mouse Anti-Human CCL18/PARC Monoclonal Antibody (Catalog # MAB394) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human CCL18/PARC Antigen Affinity-purified Polyclonal Antibody (Catalog # AF394) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)

Applications for Human CCL18/PARC Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Intracellular Staining by Flow Cytometry

2.5 µg/106 cells
Sample: Human monocyte-derived dendritic cells treated with Recombinant Human IL‑4 (Catalog # 204-IL), Recombinant Human GM-CSF (Catalog # 215-GM), Recombinant Human TNF‑ alpha (Catalog # 210-TA), Recombinant Human IL‑1 beta/IL‑1F2 (Catalog # 201-LB), and LPS, fixed with paraformaldehyde and permeabilized with saponin

Western Blot

1 µg/mL
Sample: Recombinant Human CCL18/PARC (Catalog # 394-PA)

Human CCL18/PARC Sandwich Immunoassay

ELISA Capture (Matched Antibody Pair)
Recommended Concentration: 2-8 µg/mL
Use in combination with these reagents:
  • Detection Reagent: Human CCL18/PARC Biotinylated Antibody (Catalog # BAF394)
  • Standard: Recombinant Human CCL18/PARC Protein (Catalog # 394-PA)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 4 using MAB394 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from ascites

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
Size / Price
Qty

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CCL18/PARC

CCL18, also known as alternative macrophage activation-associated CC chemokine (AMAC)-1, macrophage inflammatory protein 4 (MIP-4), and dendritic cell chemokine (DC‑CK1), is a novel CC chemokine that is highly homologous to MIP-1 alpha (61% amino acid (aa) sequence identity). CCL18 cDNA encodes an 89 aa residue precursor protein with a 20 aa putative signal peptide that is cleaved to generate a 69 aa residue mature protein which lacks potential glycosylation sites. In vitro, CCL18 mRNA expression is induced in alternatively activated macrophages by Th2 cytokines such as IL-4, IL-10, and IL-13, and inhibited by IFN-gamma. CCL18 mRNA is also expressed by GM-CSF/IL-4-induced monocyte-derived dendritic cells. In vivo, CCL18 is highly expressed in lung and placenta but is not expressed in epidermal Langerhans cells. Recombinant CCL18 has been shown to chemoattract naive T cells but not monocytes or neutrophils.

References

  1. Adema, G. et al. (1997) Nature 387:713.
  2. Kodelja, V. et al. (1998) J. Immunol. 160:141.

Alternate Names

AMAC-1, DC-CK1, MIP-4, PARC

Entrez Gene IDs

6362 (Human)

Gene Symbol

CCL18

UniProt

P55774.1

Additional CCL18/PARC Products

Product Documents for Human CCL18/PARC Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human CCL18/PARC Antibody

For research use only

Privacy Policy
Cookie Policy
Site Map
© Copyright 2026 Bio-Techne. All Rights Reserved. All trademarks and registered trademarks are the property of Bio-Techne and its brands unless otherwise specified.