Recombinant Human CCL15/MIP-1d (363-MG) chemoattracts the BaF3 mouse pro-B cell line transfected with human CCR1 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CCL15/MIP-1d (0.1 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human CCL15/MIP-1d Antigen Affinity-purified Polyclonal Antibody (Catalog # AF363). The ND₅₀ is typically 1-5 µg/mL.

Human CCL15 / MIP-1 delta ELISA Standard Curve

Human CCL15/MIP-1 delta was serially diluted and captured by Mouse Anti-Human CCL15/MIP-1 delta Monoclonal Antibody (Catalog # MAB363) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human CCL15/MIP-1 delta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF363) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)

Applications for Human CCL15/MIP-1 delta Antibody

Application

Recommended Usage

Western Blot

0.1 µg/mL
Sample: Recombinant Human CCL15/MIP-1 delta 92 aa (Catalog # 363-MG)

Neutralization

Measured by its ability to neutralize CCL15/MIP-1 delta-induced chemotaxis in the BaF3 mouse pro-B cell line transfected with human CCR1. The Neutralization Dose (ND₅₀) is typically 1-5 µg/mL in the presence of 0.1 µg/mL Recombinant Human CCL15/MIP-1 delta 92 aa.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CCL15/MIP-1 delta

CCL15, a human CC chemokine, was isolated from a human fetal spleen cDNA library. CCL15 cDNA encodes a predicted 113 amino acid (aa) protein containing a putative signal peptide of 21 aa that is cleaved to generate a 92 aa residue mature protein. Within the CC family members, human CCL15 shares 45%, 44%, 35%, and 30% aa homology with mouse C10, human MPIF-1, human HCC-1, and mouse MIP-1 gamma, respectively. The gene for MIP-1 delta is found on chromosome 17 where the genes for most of the human CC chemokines are located. Human CCL15 is expressed in T and B lymphocytes, NK cells, monocytes and monocyte-derived dendritic cells. Human MIP-1 delta is chemotactic for T cells and monocytes and has been shown to induce calcium flux in human CCR-1-transfected cells.

References

Wang, W. et al. (1998) J. Clinical Immunol. 18:214

Alternate Names

HCC-2, Leukotactin 1, MIP-1 delta, MIP-5, MIP1 delta, NCC-3

Entrez Gene IDs

6359 (Human)

Gene Symbol

CCL15

UniProt

Q16663

Additional CCL15/MIP-1 delta Products

Product Documents for Human CCL15/MIP-1 delta Antibody

Product Datasheets

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COA

SDS

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Product Specific Notices for Human CCL15/MIP-1 delta Antibody

For research use only

Citations
Reviews

Human CCL15/MIP-1 delta Antibody

R&D Systems, part of Bio-Techne | Catalog # AF363

Key Product Details

Species Reactivity

Validated:

Cited:

Applications

Validated:

Cited:

Label

Antibody Source

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Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Endotoxin Level

Scientific Data Images for Human CCL15/MIP-1 delta Antibody

Chemotaxis Induced by CCL15/MIP‑1 delta and Neutralization by Human CCL15/MIP‑1 delta Antibody.