Human Caspase-3 Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB7071
Recombinant Monoclonal Antibody.
Scientific images may be resized, cropped, or adjusted for brightness or contrast for presentation purposes. These updates are limited to presentation and do not affect the underlying scientific interpretation of the data. Where available, additional source data may be provided upon request.
Conjugate
Catalog #
Key Product Details
Validated by
Orthogonal Validation, Biological Validation
Species Reactivity
Human
Applications
Dual RNAscope ISH-IHC Compatible, Immunocytochemistry
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 1112I
Product Specifications
Immunogen
KLH-conjugated human Caspase-3 synthetic peptide
CRGTELDCGIETD
Accession # P42574
CRGTELDCGIETD
Accession # P42574
Specificity
Detects the active
form and p17 subunit of Caspase-3 in direct ELISAs, with less than 5%
cross-reactivity with the precursor form observed.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Human Caspase-3 Antibody
Caspase‑3 in Jurkat Human Cell Line.
Caspase-3 was detected in immersion fixed Jurkat human acute T cell leukemia cell line treated with Staurosporine using Rabbit Anti-Human Caspase-3 Monoclonal Antibody (Catalog # MAB7071) at 1 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rabbit IgG Secondary Antibody (red; Catalog # NL004) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Caspase-3 in Human Colon Using Dual RNAscope®ISH and IHC.
Caspase-3 mRNA was detected (top image) in formalin-fixed paraffin-embedded tissue sections of human colon probed with ACD RNAScope®Probe (Catalog # 436561) and stained using ACD RNAscope®2.5 HD Duplex Detection Reagents (red, Catalog # 322500). Adjacent tissue section (bottom image) was processed for immunohistochemistry using R&D Systems Rabbit Anti-Human Caspase-3 Monoclonal Antibody (Catalog# MAB7071) at 5 ug/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte HRP Polymer Antibody (R&D Systems, Catalog # VC001) and DAB chromogen (brown). Tissues were counterstained with hematoxylin (blue).Applications for Human Caspase-3 Antibody
Application
Recommended Usage
Dual RNAscope ISH-IHC Compatible
5-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human colon
Sample: Immersion fixed paraffin-embedded sections of human colon
Immunocytochemistry
1-25 µg/mL
Sample: Immersion fixed Jurkat human acute T cell leukemia cell line treated with Staurosporine
Sample: Immersion fixed Jurkat human acute T cell leukemia cell line treated with Staurosporine
Reviewed Applications
Read 1 review rated 5 using MAB7071 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from cell culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Caspase-3
Alternate Names
Apopain, CASP3, Caspase3, CPP32, LICE-1, YAMA
Gene Symbol
CASP3
UniProt
Additional Caspase-3 Products
Product Documents for Human Caspase-3 Antibody
Product Specific Notices for Human Caspase-3 Antibody
For research use only
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