Carbonic Anhydrase IX/CA9 was detected in immersion fixed paraffin-embedded sections of human stomach using Rabbit Anti-Human Carbonic Anhydrase IX/CA9 Monoclonal Antibody (Catalog # MAB11526) at 3 µg/ml overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using the Anti-Rabbit HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS005) or the HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm of glandular cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Applications for Human Carbonic Anhydrase IX/CA9 Antibody

Application

Recommended Usage

Immunohistochemistry

1-3 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human stomach

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute lyophilized material at 0.2mg/ml in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 μm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Carbonic Anhydrase IX/CA9

Carbonic Anhydrase (CA) catalyzes the reversible reaction of CO₂ + H₂O = HCO₃^- + H⁺, which is fundamental to many processes such as respiration, renal tubular acidification and bone resorption (1‑3). CA9, also known as membrane antigen MN and renal cell carcinoma (RCC)‑associated antigen G250, is a transmembrane enzyme expressed primarily in carcinoma cells. It is one of the best markers for hypoxia and for RCC (4, 5). The immunogen corresponds to the extracellular portion of human CA9.

References

Pastorek, J. et al. (1994) Oncogene 9: 2877.
Opavsky, R. et al. (1996) Genomics 33: 480.
Hewett-Emmett, D. and R.E. Tashian (1996) Mol. Phylogenet. Evol. 5:50.
Kaluzova, M. et al. (2004) Mol. Cell Biol. 24:5757.
Mukouyama, H. et al. (2004) Clin. Cancer Res. 10:1421.

Alternate Names

CA9, G250, MN, RCC

Entrez Gene IDs

768 (Human); 230099 (Mouse)

Gene Symbol

CA9

UniProt

Q16790

Additional Carbonic Anhydrase IX/CA9 Products

Product Documents for Human Carbonic Anhydrase IX/CA9 Antibody

Product Datasheets

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COA

SDS

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Product Specific Notices for Human Carbonic Anhydrase IX/CA9 Antibody

For research use only

Citations
Reviews

Human Carbonic Anhydrase IX/CA9 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB11526

Key Product Details

Species Reactivity

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Product Specifications

Immunogen

Specificity

Clonality

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Isotype

Scientific Data Images for Human Carbonic Anhydrase IX/CA9 Antibody

Detection of Carbonic Anhydrase IX/CA9 in Human Stomach.