Detects human Brk in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross‑reactivity with recombinant human (rh) YES, rhFyn, and rhSRC is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human Brk Antibody

Brk in Human Lymphoma.

Brk was detected in immersion fixed paraffin-embedded sections of human lymphoma using 15 µg/mL Goat Anti-Human Brk Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3966) overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Applications for Human Brk Antibody

Application

Recommended Usage

Immunohistochemistry

5-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human lymphoma subjected to Antigen Retrieval Reagent-Basic (Catalog # CTS013)

Western Blot

0.1 µg/mL
Sample: Recombinant Human Brk

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Brk

BRK (breast tumor kinase; also known as PTK6) is a 50 kDa, intracellular non-receptor tyrosine kinase. It was first identified in human breast tumor cell lines, is frequently over‑expressed in primary breast tumors, and is absent from normal breast tissue. Other sites of expression include colon, small intestine and prostate. Human BRK is 451 amino acids (aa) in length. It contains one SH3 domain (aa 12‑69), one SH2 domain (aa 77‑170) and a Tyr-kinase catalytic domain (aa 183‑444). There are two potential isoforms. The first has a 66 aa extension on the N-terminus, while the second shows the same extension plus a four aa insert after Arg 277. Over aa 1‑70, human BRK is 79% aa identical to mouse Brk.

Long Name

Breast Tumor Kinase

Alternate Names

PTK6, Sik, Tksk

Entrez Gene IDs

5753 (Human); 20459 (Mouse); 366275 (Rat)

Gene Symbol

PTK6

UniProt

Q13882

Additional Brk Products

Product Documents for Human Brk Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human Brk Antibody

For research use only

Citations
Reviews

Human Brk Antibody

R&D Systems, part of Bio-Techne | Catalog # AF3966

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

View all Brk Antibodies »

Product Specifications

Immunogen

Specificity