Human BMPR-IB/ALK-6 PE-conjugated Antibody

Detection of BMPR‑IB/ALK‑6 in PC‑3 Human Cell Line by Flow Cytometry.

PC-3 human prostate cancer cell line was stained with Mouse Anti-Human BMPR-IB/ALK-6 PE-conjugated Monoclonal Antibody (Catalog # FAB5051P, filled histogram) or isotype control antibody (Catalog # IC0041P, open histogram). View our protocol for Staining Membrane-associated Proteins.

Detection of BMPR-IB/ALK-6 in Human iPS cells differentiated to Mesoderm by Flow Cytometry.

Human iPS cells differentiated to mesoderm (using Catalog # SC030B) were stained with Mouse Anti-Human BMPR-IB/ALK-6 PE-conjugated Monoclonal Antibody (Catalog # FAB5051P, filled histogram) or isotype control antibody (Catalog # IC0041P, open histogram). View our protocol for Staining Membrane-associated Proteins.

Detection of BMPR-IB/ALK-6 by Immunohistochemistry

Characterization of BmprIB+ dermal cells. (A): Immunohistochemical staining of BmprIB in human foreskin. (B): Percentage evaluation of BmprIB expression in freshly isolated human dermal cells by flow cytometry. Histograms of BmprIB expression (left; 3.5% ± 0.4%) and isotype control (right). (C): Cell morphology under phase‐contrast microscopy. (D): The proliferative potential was assessed with the Alamar Blue assay in usDCs and BmprIB+ cells. Cell numbers were quantified by the absorbance wavelength at 570 nm using a spectrophotometer at 24, 48, 72, and 96 hours. Data represented the mean of three individuals and each of them was the mean of triplicate experiments. The osteogenic potential of BmprIB+ cells was demonstrated by ALP staining (E) and ARS staining (F). (G): The mRNA expression levels of ALP (at day 7) and OCN, OPN, and BSP (at day 21) were analyzed by real‐time polymerase chain reaction after osteogenic induction. Data represent the mean ± SD (n = 3). ∗, p <.05 indicates statistical significance. Scale bars = 50 µm. Abbreviations: ALP, alkaline phosphatase; ARS, alizarin red S stain; BmprIB, bone morphogenetic protein receptor type IB; usDC, unsorted dermal cell. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/28170187), licensed under a CC-BY license. Not internally tested by R&D Systems.