Human Bikunin Alexa Fluor® 594-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # AF7744T
Key Product Details
Species Reactivity
Applications
Label
Antibody Source
Product Specifications
Immunogen
Ala206-Asn352
Accession # P02760
Specificity
Clonality
Host
Isotype
Applications for Human Bikunin Alexa Fluor® 594-conjugated Antibody
Western Blot
Formulation, Preparation, and Storage
Purification
Formulation
Shipping
Stability & Storage
Background: Bikunin
Bikunin (also known as I alphaI light chain, EDC1, HI30 and Trypstatin) is a secreted, 35-45 kDa proteoglycan member of the kunin family of serine protease inhibitors. It is expressed by hepatocytes, amniotic epithelium and mesothelial cells. Bikunin possesses both sulfated and nonsulfated GAG sequences. The nonsulfated sequences are known to form ester linkages with two 80 kDa "heavy" chains in the hepatocyte Golgi, forming 180-200 kDa I alphaI. This molecule participates in the formation of hyaluronan-containing ECM. In addition, one of the H chains of I alphaI can be displaced by TSG6, creating an antiplasmin complex. Human bikunin is generated through cleavage of a precursor molecule termed AMBP. This AMBP should not be confused with AMBP-1, a 120-140 kDa adrenomedullin-binding protein that is also known as Complement Factor H. The AMBP precursor contains a 19 aa signal sequence, an N-terminal 183 aa A1M protein (aa 20-203), and the C-terminal serine protease inhibitor bikunin (aa 206-352). Bikunin possesses one GAG attachment site at Ser215, two Kunitz inhibitor domains (aa 231-281 and 287-337) and one utilized N-linked glycosylation site at Asn250. Although cleavage of AMBP in the Golgi may result in release of free bikunin, the 60-65 kDa AMBP precursor can also be released intact. Over aa 206-352, human bikunin shares 77% aa sequence identity with mouse bikunin.
Alternate Names
Entrez Gene IDs
Gene Symbol
UniProt
Additional Bikunin Products
Product Specific Notices for Human Bikunin Alexa Fluor® 594-conjugated Antibody
This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.
For research use only