Detects human beta-1,4-Glucuronyltransferase 1/B4GAT1 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human (rh) B3GNT2, rhB3GNT6, or rhB4GalT1 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG_2B

Scientific Data Images for Human beta-1,4-Glucuronyltransferase 1/B4GAT1 Antibody

Detection of Human beta-1,4-Glucuronyltransferase 1/B4GAT1 by Western Blot.

Western blot shows lysates of IMR-32 human neuroblastoma cell line and HEK293T human embryonic kidney cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human beta-1,4-Glucuronyltransferase 1/B4GAT1 Monoclonal Antibody (Catalog # MAB6664) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for beta-1,4-Glucuronyltransferase 1/B4GAT1 at approximately 55 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Applications for Human beta-1,4-Glucuronyltransferase 1/B4GAT1 Antibody

Application

Recommended Usage

Western Blot

2 µg/mL
Sample: IMR‑32 human neuroblastoma cell line and HEK293T human embryonic kidney cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: beta-1,4-Glucuronyltransferase 1/B4GAT1

B3GNT1 ( beta-1,3-N-acetylglucosaminyltransferase 1; also iGnT) is a 50 kD member of the b3GnT family of enzymes. It is synthesized by sensory neurons, endothelial cells and skeletal muscle. In the trans-Golgi, it collaborates with B4GALT1 to generate a linear poly-N-acetyllactosamine sequence that can be incorporated into either N-linked or O-linked glycans. Human B3GNT1 is a 451 amino acid (aa) type II transmembrane protein. It contains an eight aa N-terminal cytoplasmic segment, an extended 28 aa transmembrane region (aa 9-36), and a 379 aa luminal domain (aa 37-415). Over aa 37-415, human B3GNT1 shares 97% aa identity with mouse B3GNT1.

Long Name

beta-1,4-Glucuronyltransferase 1

Alternate Names

B3GNT1, iGAT, iGNT, MDDGA13

Entrez Gene IDs

11041 (Human); 108902 (Mouse); 293667 (Rat)

Gene Symbol

B4GAT1

UniProt

O43505 (Human)

Additional beta-1,4-Glucuronyltransferase 1/B4GAT1 Products

Product Documents for Human beta-1,4-Glucuronyltransferase 1/B4GAT1 Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human beta-1,4-Glucuronyltransferase 1/B4GAT1 Antibody

For research use only

Citations
Reviews

Human beta-1,4-Glucuronyltransferase 1/B4GAT1 Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB6664

Key Product Details

Species Reactivity

Validated:

Cited:

Applications