Human BASP1 Alexa Fluor® 532-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # AF6479X
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Western Blot
Label
Alexa Fluor 532 (Excitation = 534 nm, Emission = 553 nm)
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
E. coli-derived recombinant human BASP1
Gly2-Ala45
Accession # P80723
Gly2-Ala45
Accession # P80723
Specificity
Detects human BASP1 in Western blots.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Applications for Human BASP1 Alexa Fluor® 532-conjugated Antibody
Application
Recommended Usage
Western Blot
Optimal dilution of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
Background: BASP1
BASP1 (Brain acid soluble protein 1; also NAP-22) is a transcriptional cosuppressor that belongs to the BASP1 family of proteins. Although its predicted MW is 23 kDa, BASP1 will show multiple bands in SDS-Page. First, BASP1 forms oligomers in SDS, ranging in size from 30-150 kDa. Second, varying concentrations of SDS will change the apparent BASP1 MW from 56 kDa in 8% SDS-Page to 41 kDa in 13% SDS-Page. At least two forms of BASP1 apparently exist in human, one that is 48-52 kDa, and another that is 32-40 kDa in size. They are not functionally equivalent. BASP1 can be SUMOylated that add 20 kDa in apparent MW. There are also N-terminal fragments termed BIRPs that run from 30-50 kDa in 12% acidic SDS-Page. BASP1 is expressed in neurons, renal podocytes and spermatids. It binds to the cytoplasmic side of the plasmalemma via its myristroylate adduct, and undergoes nuclear translocation when SUMOylated. Human BASP1 is 227 amino acids (aa) in length. It contains a myristoylation site at Gly2, SUMOylation sites at Lys79 and 84, at least eight utilized phosphorylation sites, and an NLS. One splice variant shows a deletion of aa 88-141. Over aa 1-45, human BASP1 shares 96% and 91% aa identity with mouse and rat BASP1, respectively.
Long Name
Brain Acid Soluble Protein 1
Alternate Names
CAP23, NAP22
Gene Symbol
BASP1
UniProt
Additional BASP1 Products
Product Specific Notices for Human BASP1 Alexa Fluor® 532-conjugated Antibody
This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.
For research use only
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