Human B7-1/CD80 Antibody Best Seller

B7-1/CD80 in Human Tonsil Using Dual RNAscope^®ISH and IHC.

B7-1/CD80 mRNA was detected in formalin-fixed paraffin-embedded tissue sections of human tonsil probed with ACD RNAScope^®Probe (Catalog # 42471) and stained using ACD RNAscope^®2.5 HD Duplex Detection Reagents (red, Catalog # 322500). Adjacent tissue section was processed for immunohistochemistry using R&D Systems Mouse Anti-Human B7-1/CD80 Monoclonal Antibody (Catalog# MAB140) at 5ug/mL with overnight incubation at 4 °C followed by incubation with the Anti-Mouse IgG VisUCyte HRP Polymer Antibody (R&D Systems, Catalog # VC001) and DAB chromogen (lower image, brown). Tissues were counterstained with hematoxylin (blue).

Detection of Mouse B7-1/CD80 by Immunohistochemistry

MNK inhibitors induce polarization of TAMs toward an M2 phenotype in ex vivo human PDAC slice cultures.(A) Serial sections of human PDAC tumors (n = 15) were stained for Arginase-1 and phosphorylated eIF4ES209 (p-eIF4E) by IHC. Scale bar: 200 μm. The staining positivity was analyzed by the TissueGnostics Imaging system using the HistoQuest, and the correlation analysis for Arginase-1 and p-eIF4E expression was performed using GraphPad. (B) Slice cultures (n = 1–4 replicates for each treatment group) from 6 different PDAC tumors were treated with DMSO (vehicle control) or CGP57380 for 5 days and stained for phosphorylated eIF4E (p-eIF4E), CD68, Arginase-1, CD163, MRC1, CD86, CD80, and MHCII. The percentage of positive cells, relative to the total number of nucleated cells, was analyzed by ImageJ. Scale bar: 50 μm. Data points represent individual patients. Paired t test was performed by GraphPad. *P < 0.05; **P < 0.01. (C) The relative expression of MKNK2, CD163, and MRC1 in TCGA studies and their transcript abundance from RNA-Seq data, quantified as RSEM, were downloaded from cBioPortal. Correlation analysis was performed in GraphPad to evaluate the relationship between MKNK2, CD163, and MRC1. Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/35380995), licensed under a CC-BY license. Not internally tested by R&D Systems.

Human B7-1 / CD80 ELISA Standard Curve

Recombinant Human B7-1/CD80 Fc Chimera (Catalog # 140-B1) was serially diluted and captured by Mouse Anti-Human B7-1/CD80 Monoclonal Antibody (Catalog # MAB140) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human B7-1/CD80 Biotinylated Monoclonal Antibody (Catalog # ) was incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)