Human alpha1-Microglobulin Alexa Fluor® 594-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # AF7724T
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Western Blot
Label
Alexa Fluor 594 (Excitation = 590 nm, Emission = 617 nm)
Antibody Source
Polyclonal Sheep IgG
Product Specifications
Immunogen
E. coli-derived recombinant human α1-Microglobulin
Gly20-Val203, predicted
Accession # P02760
Gly20-Val203, predicted
Accession # P02760
Specificity
Detects human alpha1-Microglobulin in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant rat alpha1-Microglobulin is observed.
Clonality
Polyclonal
Host
Sheep
Isotype
IgG
Applications
Application
Recommended Usage
Western Blot
Optimal dilution of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
Background: alpha 1-Microglobulin
Human alpha1-Microglobulin (alpha1-m/A1M; also protein HC) is a secreted, 31-32 kDa glycoprotein member of the lipocalin family, calycin superfamily of molecules. It is expressed by hepatocytes, keratinocytes, and endodermal derivatives in the embryo. A1M appears to act as a heme scavenger, protecting cells and collagen against oxidative damage. It also acts as an immunosuppressant, inhibiting polyclonal lymphocyte activation and dampening granulocyte migration in response to chemokines. A1M circulates either as a monomer, or bound to IgA, albumin or prothrombin. Human A1M is generated through cleavage of a precursor molecule termed AMBP. This AMBP should not be confused with AMBP-1, a 120-140 kDa adrenomedullin-binding protein that is also known as Complement Factor H. The AMBP precursor contains a 19 aa signal sequence, an N-terminal 183 aa A1M protein (aa 20-203), and a C-terminal serine protease inhibitor termed bikunin (aa 206-352). A1M possesses one lipocalin domain (aa 42-186). Although cleavage of AMBP in the Golgi apparatus typically generates a 31 kDa A1M and 28 kDa bikunin molecule, the 60-65 kDa AMBP precursor can also be released intact. A1M will undergo extracellular processing, generating a 30 kDa isoform that is missing aa 199-203. There is one splice variant that shows a deletion of aa 48-57. Over aa 20-203, human A1M shares 76% aa sequence identity with both mouse and rat A1M.
Alternate Names
A1M, alpha 1Microglobulin, AMBP, EDC1, HCP, HI30, IATIL, ITI, ITIL, ITILC, UTI
Gene Symbol
AMBP
UniProt
Additional alpha 1-Microglobulin Products
Product Specific Notices
This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.
For research use only
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