Human alpha2-Macroglobulin Antibody
R&D Systems, part of Bio-Techne | Catalog # MAB1938
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human, Canine
Applications
Validated:
Western Blot, Simple Western
Cited:
Western Blot, Simple Western, ELISA Control
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 257316
Product Specifications
Immunogen
Human plasma-derived alpha2-Macroglobulin
Specificity
Detects human alpha2-Macroglobulin in direct ELISAs and Western blots. In direct ELISAs, this antibody does not cross-react with rhC5a or rmC5a.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for Human alpha2-Macroglobulin Antibody
Detection of Human alpha2‑Macroglobulin by Western Blot.
Western blot shows human serum. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human a2-Macroglobulin Monoclonal Antibody (Catalog # MAB1938) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for a2-Macroglobulin at approximately 180 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Human alpha2-Macroglobulin by Simple WesternTM.
Simple Western lane view shows lysates of Exosome Standards (HT-29) (NBP3-11685) and human serum, loaded at 0.5 mg/ml and 1:10 respectively. A specific band was detected for alpha2-Macroglobulin at approximately 178 kDa (as indicated) using 10 µg/ml of Mouse Anti-Human alpha2-Macroglobulin Monoclonal Antibody (Catalog # MAB1938) followed by HRP-conjugated Goat Anti-Mouse Secondary Antibody (Catalog # 042-205). This experiment was conducted under reducing conditions and using the 12-230kDa separation system.
Detection of Human alpha2‑Macroglobulin by Simple WesternTM.
Simple Western lane view shows human serum, loaded at 0.2 mg/mL. A specific band was detected for alpha2‑Macroglobulin at approximately 178 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human alpha2‑Macroglobulin Monoclonal Antibody (Catalog # MAB1938) . This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.Applications for Human alpha2-Macroglobulin Antibody
Application
Recommended Usage
Simple Western
10 µg/mL
Sample: Exosome Standards (HT-29) (Catalog # NBP3-11685) and Human serum
Sample: Exosome Standards (HT-29) (Catalog # NBP3-11685) and Human serum
Western Blot
2 µg/mL
Sample: Human serum
Sample: Human serum
Reviewed Applications
Read 3 reviews rated 3.7 using MAB1938 in the following applications:
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: alpha 2-Macroglobulin
Alpha 2-macroglobulin (A2M) is a general and irreversible protease inhibitor implicated in many processes. It is able to inhibit all four classes of proteases by a unique trapping mechanism.
Additional alpha 2-Macroglobulin Products
Product Documents for Human alpha2-Macroglobulin Antibody
Product Specific Notices for Human alpha2-Macroglobulin Antibody
For research use only
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