Human alpha-1,4-N-Acetylglucosaminyltransferase 4/A4GNT Antibody
R&D Systems, part of Bio-Techne | Catalog # AF8555
Key Product Details
Species Reactivity
Human
Applications
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Product Specifications
Immunogen
Chinese hamster ovary cell line CHO-derived recombinant human alpha-1,4-N-Acetylglucosaminyltransferase 4/A4GNT
Leu25-Lys340
Accession # Q9UNA3
Leu25-Lys340
Accession # Q9UNA3
Specificity
Detects human alpha-1,4-N-Acetylglucosaminyltransferase in direct ELISAs and Western blots.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Human alpha-1,4-N-Acetylglucosaminyltransferase 4/A4GNT Antibody
Detection of Human alpha-1,4-N-Acetylglucosaminyltransferase 4/A4GNT by Western Blot.
Western blot shows lysates of SW480 human colorectal adenocarcinoma cell line, COLO 205 human colorectal adenocarcinoma cell line, KATO-III human gastric carcinoma cell line, and HT-29 human colon adenocarcinoma cell line. PVDF membrane was probed with 1:1000 dilution of Rabbit Anti-Human a-1,4-N-Acetylglucosaminyltransferase 4/A4GNT Antigen Affinity-purified Polyclonal Antibody (Catalog # AF8555) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for a-1,4-N-Acetylglucosaminyltransferase 4/A4GNT at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Human alpha-1,4-N-Acetylglucosaminyltransferase 4/A4GNT Antibody
Application
Recommended Usage
Western Blot
1:1000 dilution
Sample: SW480 human colorectal adenocarcinoma cell line, COLO 205 human colorectal adenocarcinoma cell line, KATO‑III human gastric carcinoma cell line, and HT‑29 human colon adenocarcinoma cell line
Sample: SW480 human colorectal adenocarcinoma cell line, COLO 205 human colorectal adenocarcinoma cell line, KATO‑III human gastric carcinoma cell line, and HT‑29 human colon adenocarcinoma cell line
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified
Formulation
Supplied as a solution in PBS containing BSA, Glycerol and Sodium Azide.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
- 12 months from date of receipt, -20 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after opening.
- 6 months, -20 °C under sterile conditions after opening.
Background: Alpha-1,4-N-Acetylglucosaminyltransferase 4/A4GNT
Alpha-1,4-N-Acetylglucosaminyltransferase 4, also known as A4GNT is a 45 kDa glycoprotein, member of the glycosyltransferase 32 family of proteins. A4GNT catalyzes the transfer of N-acetylglucosamine (GlcNAc) to core 2 branched O-glycans. A4GNT is a single-pass type II membrane protein associated with the Golgi apparatus and contains the conserved DXD motif involved in catalytic activity. It is expressed in stomach and pancreas, as well as in gastric cancer cells. A4GNT is not expressed in peripheral blood cells, making it a putative biomarker for pancreatic cancer. A4GNT and Mucin 6 expression is upregulated in the gastric mucosa of H. pylori infected patients. Lumenal domain of human A4GNT shares 72% aa sequence identity with mouse and rat A4GNT.
Alternate Names
alpha4GnT
Gene Symbol
A4GNT
UniProt
Additional Alpha-1,4-N-Acetylglucosaminyltransferase 4/A4GNT Products
Product Documents for Human alpha-1,4-N-Acetylglucosaminyltransferase 4/A4GNT Antibody
Product Specific Notices for Human alpha-1,4-N-Acetylglucosaminyltransferase 4/A4GNT Antibody
* Contains <0.1% Sodium Azide, which is not hazardous at this concentration according to GHS classifications. Refer to SDS for additional information and handling instructions.
For research use only
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