Key Product Details

Species Reactivity

Human

Applications

Intracellular Staining by Flow Cytometry, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 199822

View all Akt3 Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Akt3
Arg23-Ala101
Accession # NP_005456

Specificity

Detects human Akt3 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) Akt1 or rhAkt2 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Applications for Human Akt3 Antibody

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Intracellular Staining by Flow Cytometry

2.5 µg/106 cells
Sample: MCF-7 human breast cancer cell line fixed with paraformaldehyde and permeabilized with saponin

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Reconstitution Buffer Available:
Size / Price
Qty

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Akt3

Akt3 (v-akt murine thymoma viral oncogene homolog 3), also known as protein kinase B gamma (PKB gamma) and RAC-gamma serine/threonine kinase (RAC-PK-gamma), is a member of the Akt family that also includes Akt1 (PKB alpha) and Akt2 (PKB beta). These highly homologous kinases are central in such diverse cellular processes as glucose uptake, cell cycle progression, and apoptosis. All three Akts contain an amino-terminal pleckstrin homology domain, a central kinase domain, and a carboxyl-terminal regulatory domain.

Long Name

v-Akt Murine Thymoma Viral Oncogene Homolog 3

Alternate Names

PKB gamma, RAC-gamma

Entrez Gene IDs

10000 (Human); 23797 (Mouse)

Gene Symbol

AKT3

UniProt

NP_005456

Additional Akt3 Products

Product Documents for Human Akt3 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human Akt3 Antibody

For research use only

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