Human Airway Trypsin-like Protease/HAT Alexa Fluor™ Plus 647-conjugated Antibody
R&D Systems, part of Bio-Techne | Catalog # FAB2695AFP647
Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Western Blot
Label
Alexa Fluor Plus 647 (Excitation = 658 nm, Emission = 675 nm)
Antibody Source
Monoclonal Mouse IgG1 Clone # 337029
Product Specifications
Specificity
Detects human Airway Trypsin-like Protease/HAT in direct ELISAs and Western blots. Recognizes the catalytic-domain of recombinant human HAT.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Applications
Application
Recommended Usage
Western Blot
Optimal dilution of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Formulation
Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
Background: Airway Trypsin-like Protease/HAT
Human Airway Trypsin‑like protease (HAT), also known as Transmembrane Protease, Serine 11D (TMPRSSIID), is a type II transmembrane serine protease that is expressed in the cells of the submucosal serous glands of the bronchi and trachea (1‑3). The deduced sequence predicts a short cytoplasmic tail (aa 120), a transmembrane domain (aa 21‑41), and an ectodomain consisting of a SEA domain (aa 44‑164) and a catalytic domain (aa 187‑418). The single‑chain precursor can be converted into a disulfide‑linked two‑chains, one of which corresponds to the catalytic domain. HAT was initially purified from the sputum of patients with chronic airway diseases (1). HAT has been shown to induce PAR‑2 mediated IL‑8 release in psoriasis vulgaris and increase mucin expression in airway epithelial cells (4, 5). The amino acid sequence of human HAT is 99%, 79%, and 67% identical to that of chimpanzee, canine and rat/mouse.
References
- Yasuoka, S. et al. (1997) Am. J. Respir. Cell Mol. Biol. 16:300.
- Yamaoka, K. et al. (1998) J. Biol. Chem. 273:11895.
- Hooper, J.D. et al. (2001) J. Biol. Chem. 276:857.
- Iwakiri, K. et al. (2004) J. Invest. Dermatol. 122:937.
- Chokki, M. et al. (2004) Am. J. Respir. Cell Mol. Biol. 30:470.
Long Name
Human Airway Trypsin-like Protease
Alternate Names
TMPRSS11D
Gene Symbol
TMPRSS11D
UniProt
Additional Airway Trypsin-like Protease/HAT Products
Product Specific Notices
This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.
For research use only
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