A172 human glioblastoma cell line was stained with Mouse Anti-Human AATK Monoclonal Antibody (Catalog # MAB4866, filled histogram) or isotype control antibody (Catalog # MAB0041, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG F(ab')₂Secondary Antibody (Catalog # F0102B). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.

Applications for Human AATK Antibody

Application

Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Intracellular Staining by Flow Cytometry

0.25 µg/10⁶ cells
Sample: A172 human glioblastoma cell line fixed with paraformaldehyde and permeabilized with saponin

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: AATK

Apoptosis-associated tyrosine kinase (AATK) is a 155 kDa membrane-associated intracellular protein that promotes neurite extension and neuronal differentiation. Low extracellular potassium levels induce AATK phosphorylation and apoptosis in cerebellar granule cells. AATK negatively regulates the Na-K-2Cl cotransporter by promoting its PP1-dependent dephosphorylation. The two alternate splice forms of AATK also contain the sequences included in this immunogen. Within this region, human AATK shares 63% aa sequence identity with mouse AATK.

Long Name

Apoptosis-associated Tyrosine Kinase

Alternate Names

AATYK, LMR1, LMTK1, p35BP

Entrez Gene IDs

9625 (Human); 11302 (Mouse); 690853 (Rat)

Gene Symbol

AATK

UniProt

AAH47378

Additional AATK Products

Product Documents for Human AATK Antibody

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for Human AATK Antibody

For research use only

Citations
Reviews

Calculators
Flow Cytometry

Human AATK Antibody

R&D Systems, part of Bio-Techne | Catalog # MAB4866

Key Product Details

Species Reactivity

Applications

Label

Antibody Source

View all AATK Antibodies »

Product Specifications

Immunogen

Specificity

Clonality

Host

Isotype

Scientific Data Images for Human AATK Antibody

Detection of AATK in A172 Human Cell Line by Flow Cytometry.