HSPB8/HSP22 Antibody (3K10W1)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15464
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
ELISA, Immunocytochemistry/ Immunofluorescence, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 3K10W1 expressed in HEK293
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human HSPB8/HSP22 (Q9UJY1). MADGQMPFSCHYPSRLRRDPFRDSPLSSRLLDDGFGMDPFPDDLTASWPDWALPRLSSAWPGTLRSGMVPRGPTATARFGVPAEGRTPPPFPGEPWKVCV
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
22 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
Novus Biologicals Rabbit HSPB8/HSP22 Antibody (3K10W1) (NBP3-15464) is a recombinant monoclonal antibody validated for use in WB, ELISA and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for HSPB8/HSP22 Antibody (3K10W1)
![Western Blot: HSPB8/HSP22 Antibody (3K10W1) [NBP3-15464]](https://resources.bio-techne.com/images/products/HSPB8-HSP22-Antibody-3K10W1-Western-Blot-NBP3-15464-img0003.jpg "Western Blot: HSPB8/HSP22 Antibody (3K10W1) [NBP3-15464]")
Western Blot: HSPB8/HSP22 Antibody (3K10W1) [NBP3-15464]
Western Blot: HSPB8/HSP22 Antibody (3K10W1) [NBP3-15464] - Western blot analysis of extracts of various cell lines, using HSPB8/HSP22 Rabbit mAb (NBP3-15464) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.
Immunocytochemistry/ Immunofluorescence: HSPB8/HSP22 Antibody (3K10W1) [NBP3-15464] -
Immunocytochemistry/ Immunofluorescence: HSPB8/HSP22 Antibody (3K10W1) [NBP3-15464] - Immunofluorescence analysis of NIH-3T3 cells using HSPB8/HSP22 Rabbit mAb at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunocytochemistry/ Immunofluorescence: HSPB8/HSP22 Antibody (3K10W1) [NBP3-15464] -
Immunocytochemistry/ Immunofluorescence: HSPB8/HSP22 Antibody (3K10W1) [NBP3-15464] - Immunofluorescence analysis of U-2 OS cells using HSPB8/HSP22 Rabbit mAb at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.Applications for HSPB8/HSP22 Antibody (3K10W1)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:50 - 1:200
Western Blot
1:500 - 1:1000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.3), 50% glycerol, 0.05% BSA
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: HSPB8
Long Name
Heat Shock Protein beta 8
Alternate Names
CMT2L, CRYAC, DHMN2, E2IG1, H11, HMN2A, HSP22
Gene Symbol
HSPB8
Additional HSPB8 Products
Product Documents for HSPB8/HSP22 Antibody (3K10W1)
Product Specific Notices for HSPB8/HSP22 Antibody (3K10W1)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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