HSP70/HSPA1A Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-77455
![Flow Cytometry: HSP70/HSPA1A Antibody - BSA Free [NBP1-77455]](https://resources.bio-techne.com/images/products/HSP70-HSPA1A-Antibody-Flow-Cytometry-NBP1-77455-img0011.jpg "Flow Cytometry: HSP70/HSPA1A Antibody - BSA Free [NBP1-77455]")
![Immunocytochemistry/ Immunofluorescence: HSP70/HSPA1A Antibody - BSA Free [NBP1-77455]](https://resources.bio-techne.com/images/products/HSP70-HSPA1A-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-77455-img0009.jpg "Immunocytochemistry/ Immunofluorescence: HSP70/HSPA1A Antibody - BSA Free [NBP1-77455]")
Scientific images may be resized, cropped, or adjusted for brightness or contrast for presentation purposes. These updates are limited to presentation and do not affect the underlying scientific interpretation of the data. Where available, additional source data may be provided upon request.
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human, Mouse
Cited:
Mouse
Predicted:
Bovine (100%), Canine (100%), Porcine (100%), Primate (100%), Rat (92%). Backed by our 100% Guarantee.
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Simple Western
Cited:
Immunohistochemistry-Frozen, Flow Cytometry, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
1.18 mg/ml
Product Specifications
Immunogen
A synthetic peptide made to an internal portion of the human Hsp70 protein (between residues 550-600)
Localization
Cytoplasm. Note: Localized in cytoplasmic mRNP granules containing untranslated mRNAs.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for HSP70/HSPA1A Antibody - BSA Free
Immunocytochemistry/ Immunofluorescence: HSP70/HSPA1A Antibody - BSA Free [NBP1-77455]
Immunocytochemistry/Immunofluorescence: HSP70/HSPA1A Antibody [NBP1-77455] - HSP70 antibody was tested at 1:25 in HeLa cells with FITC (green). Nuclei were counterstained with DAPI (blue).![Immunohistochemistry: HSP70/HSPA1A Antibody - BSA Free [NBP1-77455]](https://resources.bio-techne.com/images/products/HSP70-HSPA1A-Antibody-Immunohistochemistry-NBP1-77455-img0010.jpg "Immunohistochemistry: HSP70/HSPA1A Antibody - BSA Free [NBP1-77455]")
Immunohistochemistry: HSP70/HSPA1A Antibody - BSA Free [NBP1-77455]
Immunohistochemistry: HSP70/HSPA1A Antibody [NBP1-77455] - Analysis of Hsp70 in human xenograft kidney cancer using DAB with hematoxylin counterstain.![Flow Cytometry: HSP70/HSPA1A Antibody - BSA Free [NBP1-77455]](https://resources.bio-techne.com/images/products/HSP70-HSPA1A-Antibody-Flow-Cytometry-NBP1-77455-img0014.jpg "Flow Cytometry: HSP70/HSPA1A Antibody - BSA Free [NBP1-77455]")
Flow Cytometry: HSP70/HSPA1A Antibody - BSA Free [NBP1-77455]
Flow Cytometry: HSP70/HSPA1A Antibody [NBP1-77455] - An intracellular stain was performed on Jurkat cells with HSP70/HSPA1A Antibody NBP1-77455AF488 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Cells were incubated in an antibody dilution of 10 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488.![Flow Cytometry: HSP70/HSPA1A Antibody - BSA Free [NBP1-77455]](https://resources.bio-techne.com/images/products/HSP70-HSPA1A-Antibody-Flow-Cytometry-NBP1-77455-img0013.jpg "Flow Cytometry: HSP70/HSPA1A Antibody - BSA Free [NBP1-77455]")
![Simple Western: HSP70/HSPA1A AntibodyBSA Free [NBP1-77455]](https://resources.bio-techne.com/images/products/HSP70-HSPA1A-Antibody-Simple-Western-NBP1-77455-img0012.jpg "Simple Western: HSP70/HSPA1A AntibodyBSA Free [NBP1-77455]")
Applications for HSP70/HSPA1A Antibody - BSA Free
Application
Recommended Usage
Flow Cytometry
1 : 200
Immunocytochemistry/ Immunofluorescence
1:25
Immunohistochemistry
1:400
Immunohistochemistry-Frozen
reported in scientific literature (PMID 26755821)
Immunohistochemistry-Paraffin
1:400
Simple Western
1:50
Application Notes
In Simple Western only 10 - 15 uL of the recommended dilution is used per data point.
See Simple Western Antibody Database for Simple Western validation: Tested in HeLa lysate 1.0 mg/mL, separated by Size, antibody dilution of 1:50, apparent MW was 67 kDa. Separated by Size-Wes, Sally Sue/Peggy Sue.
See Simple Western Antibody Database for Simple Western validation: Tested in HeLa lysate 1.0 mg/mL, separated by Size, antibody dilution of 1:50, apparent MW was 67 kDa. Separated by Size-Wes, Sally Sue/Peggy Sue.
Reviewed Applications
Read 1 review rated 3 using NBP1-77455 in the following applications:
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS and 30% Glycerol
Format
BSA Free
Preservative
0.05% Sodium Azide
Concentration
1.18 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: HSP70/HSPA1A
Long Name
Heat Shock Protein 70
Alternate Names
HSP70-1A, HSP72, HSPA1A
Gene Symbol
HSPA1A
UniProt
Additional HSP70/HSPA1A Products
Product Documents for HSP70/HSPA1A Antibody - BSA Free
Product Specific Notices for HSP70/HSPA1A Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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