HSP27 Antibody (HSPB1/7038R) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-49663

Recombinant Monoclonal Antibody
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NBP3-49663
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Kappa Clone # HSPB1/7038R

Format

Azide and BSA Free

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all HSP27 Antibodies »

Product Specifications

Immunogen

Recombinant human full-length HSP27 protein (Uniprot: P04792)

Localization

Cytoplasm, Cytoskeleton, Nucleus, Spindle

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG Kappa

Theoretical MW

27 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

Prepared in 10mM PBS WITHOUT BSA & azide at 1.0mg/ml.

Antibody without azide - store at -20 to -80C. Antibody is stable for 24 months. Non-hazardous.

Scientific Data Images for HSP27 Antibody (HSPB1/7038R) - Azide and BSA Free

HSP27 Antibody (HSPB1/7038R) - Azide and BSA Free

Immunohistochemistry-Paraffin: HSP27 Antibody (HSPB1/7038R) - Azide and BSA Free [NBP3-49663] -

Formalin-fixed, paraffin-embedded human prostate stained with HSP27 Antibody (HSPB1/7038R) - Azide and BSA Free. HIER: Tris/EDTA, pH9.0, 45min. 2�C: HRP-polymer, 30min. DAB, 5min.
HSP27 Antibody (HSPB1/7038R) - Azide and BSA Free

Immunohistochemistry-Paraffin: HSP27 Antibody (HSPB1/7038R) - Azide and BSA Free [NBP3-49663] -

Formalin-fixed, paraffin-embedded human renal cell carcinoma (RCC) stained with HSP27 Antibody (HSPB1/7038R) - Azide and BSA Free. HIER: Tris/EDTA, pH9.0, 45min. 2�: HRP-polymer, 30min. DAB, 5min.
HSP27 Antibody (HSPB1/7038R) - Azide and BSA Free

Western Blot: HSP27 Antibody (HSPB1/7038R) - Azide and BSA Free [NBP3-49663] -

Western blot analysis of human liver and MCF7 lysates using HSP27 Antibody (HSPB1/7038R) - Azide and BSA Free.

Applications for HSP27 Antibody (HSPB1/7038R) - Azide and BSA Free

Application
Recommended Usage

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -80C. Avoid freeze-thaw cycles.

Background: HSP27

Hsp25 is the mouse homologue of the human Hsp27 protein, a member of the small heat shock protein family comprised of a diverse group of proteins from approx. 15 to >30kDa(1). The basic structure of most sHsps is a homologous and highly conserved amino acid sequence, with an alpha-crystallin-domain at the C-terminus and the WD/EPF domain at the less conserved N-terminus. This N-terminus is essential for the development of high molecular oligomers (2, 3). Hsp27-oligomers consist of stable dimers formed by as many as 8-40 Hsp27 protein monomers (4). The oligomerization status is connected with the chaperone activity: aggregates of large oligomers have high chaperone activity, whereas dimers have no chaperone activity (5). HSP27 is localized to the cytoplasm of unstressed cells but can redistribute to the nucleus in response to stress, where it may function to stabilize DNA and/or the nuclear membrane. It can be rapidly phosphorylated in response to physiological stimuli relevant to the cell type examined. Thus, hsp27 has been suggested to be an important intermediate in second messenger mediated signaling pathways (6). Other functions include chaperone activity (as mentioned above), thermotolerance in vivo, inhibition of apoptosis, and signal transduction. Specifically, in vitro, it acts as an ATP-independent chaperone by inhibiting protein aggregation and by stabilizing partially denatured proteins, which ensures refolding of the HSP70 complex. Hsp27 is also involved in the apoptotic signaling pathway because it interferes with the activation of cytochrome c/Apaf-1/dATP complex, thereby inhibiting the activation of procaspase-9. It is also hypothesized that hsp27 may serve some role in cross-bridge formation between actin and myosin (7). And finally, Hsp27 is also thought to be involved in the process of cell differentiation. The up-regulation of Hsp27 correlates with the rate of phosphorylation and with an increase of large oligomers. It is possible that Hsp27 may play a crucial role in termination of growth (8).

Long Name

Heat Shock Protein 27

Alternate Names

HSP25, HSPB1

Gene Symbol

HSPB1

Additional HSP27 Products

Product Documents for HSP27 Antibody (HSPB1/7038R) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for HSP27 Antibody (HSPB1/7038R) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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