HSP27 Antibody (8A7) [CoraFluor™ 1]

Novus Biologicals, part of Bio-Techne | Catalog # NB110-96431CL1

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Product Datasheet / COA / SDS

View Available Conjugates View Available Formulations

Conjugate

Catalog #

Alexa Fluor 350

NB110-96431AF350

Alexa Fluor 405

NB110-96431AF405

Alexa Fluor 488

NB110-96431AF488

Alexa Fluor 532

NB110-96431AF532

Alexa Fluor 594

NB110-96431AF594

Alexa Fluor 647

NB110-96431AF647

Alexa Fluor 700

NB110-96431AF700

Alexa Fluor 750

NB110-96431AF750

Alexa Fluor Plus 405

NB110-96431AFP405

Alexa Fluor Plus 488

NB110-96431AFP488

Alexa Fluor Plus 555

NB110-96431AFP555

Alexa Fluor Plus 594

NB110-96431AFP594

Alexa Fluor Plus 647

NB110-96431AFP647

Alexa Fluor Plus 680

NB110-96431AFP680

Allophycocyanin

NB110-96431APC

Biotin

NB110-96431B

DyLight 350

NB110-96431UV

DyLight 405

NB110-96431V

DyLight 488

NB110-96431G

DyLight 550

NB110-96431R

DyLight 594

NB110-96431DL594

DyLight 650

NB110-96431C

DyLight 680

NB110-96431FR

DyLight 755

NB110-96431IR

FITC

NB110-96431F

HRP

NB110-96431H

Janelia Fluor 525

NB110-96431JF525

Janelia Fluor 549

NB110-96431JF549

Janelia Fluor 646

NB110-96431JF646

Janelia Fluor 669

NB110-96431JF669

mFluor Violet 450 SE

NB110-96431MFV450

mFluor Violet 500 SE

NB110-96431MFV500

mFluor Violet 610 SE

NB110-96431MFV610

PE

NB110-96431PE

PerCP

NB110-96431PCP

Forumulation

Catalog #

BSA Free

NB110-96431

Product Specifications
Scientific Data
Applications
Spectra Viewer
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Human, Mouse, Rat, Bovine, Canine, Guinea Pig, Hamster, Sheep

Applications

Knockout Validated, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

CoraFluor 1

Antibody Source

Monoclonal Mouse IgG₁ kappa Clone # 8A7

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all HSP27 Antibodies »

Product Specifications

Immunogen

HSP27 peptide

Localization

Cytoplasm , Nucleus , Cytoskeleton , Spindle

Specificity

Detects approx 25kDa or approx 27kDa. Recognizes HSP25 and HSP27, cross reacts with alpha B crystallin.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁ kappa

Description

This conjugate is made on demand and is not held in inventory. Each order represents a unique lot. All conjugates are generated using validated conjugation protocols and meet strict degree of labeling (F/P) specifications; additional information is available upon request. The antibody concentration is provided on the individual product label.

For specialized conjugation needs, like large quantities, specific concentrations, and defined F/P ratios, bulk and custom antibody conjugation services are available.

Scientific Data Images for HSP27 Antibody (8A7) [CoraFluor™ 1]

Product Feature: CoraFluor Probes for TR-FRET

CoraFluor™ 1, amine reactive (Catalog:7920) and CoraFluor™ 2, amine reactive (Catalog # 7950) are terbium-based probes that have been developed for use as TR-FRET donors. They emit wavelengths compatible with commonly used fluorescent acceptor dyes such as BODIPY® (or BDY) and Janelia Fluor® dyes, FITC (Catalog # 5440), TMR and Cyanine 5 (Catalog # 5436). CoraFluor™ fluorescence is brighter and more stable in biological media than existing TR-FRET donors, leading to enhanced sensitivity and improved data generation. CoraFluor™ 1 exhibits excitation upon exposure to a 337 nm UV laser.

Applications for HSP27 Antibody (8A7) [CoraFluor™ 1]

Application

Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Immunoprecipitation

Optimal dilutions of this antibody should be experimentally determined.

Knockout Validated

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Application Notes

Recommended applications are based on validated applications from the unconjugated base product NB110-96431. This conjugated antibody is not kept in inventory and is made to order using validated conjugation protocols.

Spectra Viewer

Plan Your Experiments

Use our spectra viewer to interactively plan your experiments, assessing multiplexing options. View the excitation and emission spectra for our fluorescent dye range and other commonly used dyes.

Spectra Viewer

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark. Do not freeze.

Background: HSP27

Hsp25 is the mouse homologue of the human Hsp27 protein, a member of the small heat shock protein family comprised of a diverse group of proteins from approx. 15 to >30kDa(1). The basic structure of most sHsps is a homologous and highly conserved amino acid sequence, with an alpha-crystallin-domain at the C-terminus and the WD/EPF domain at the less conserved N-terminus. This N-terminus is essential for the development of high molecular oligomers (2, 3). Hsp27-oligomers consist of stable dimers formed by as many as 8-40 Hsp27 protein monomers (4). The oligomerization status is connected with the chaperone activity: aggregates of large oligomers have high chaperone activity, whereas dimers have no chaperone activity (5). HSP27 is localized to the cytoplasm of unstressed cells but can redistribute to the nucleus in response to stress, where it may function to stabilize DNA and/or the nuclear membrane. It can be rapidly phosphorylated in response to physiological stimuli relevant to the cell type examined. Thus, hsp27 has been suggested to be an important intermediate in second messenger mediated signaling pathways (6). Other functions include chaperone activity (as mentioned above), thermotolerance in vivo, inhibition of apoptosis, and signal transduction. Specifically, in vitro, it acts as an ATP-independent chaperone by inhibiting protein aggregation and by stabilizing partially denatured proteins, which ensures refolding of the HSP70 complex. Hsp27 is also involved in the apoptotic signaling pathway because it interferes with the activation of cytochrome c/Apaf-1/dATP complex, thereby inhibiting the activation of procaspase-9. It is also hypothesized that hsp27 may serve some role in cross-bridge formation between actin and myosin (7). And finally, Hsp27 is also thought to be involved in the process of cell differentiation. The up-regulation of Hsp27 correlates with the rate of phosphorylation and with an increase of large oligomers. It is possible that Hsp27 may play a crucial role in termination of growth (8).

Long Name

Heat Shock Protein 27

Alternate Names

HSP25, HSPB1

Gene Symbol

HSPB1

Additional HSP27 Products

Product Documents for HSP27 Antibody (8A7) [CoraFluor™ 1]

Product Datasheets

Download Product Datasheets

COA

SDS

Product Specific Notices for HSP27 Antibody (8A7) [CoraFluor™ 1]

Sold for research purposes only under agreement from Massachusetts General Hospital, US Patent No. 12,134,721. A license is required for development of new commercial assay platforms (e.g., microarrays, microfluidics), diagnostic use or other unlicensed uses. Please contact Technical Support for further details: techsupport@bio-techne.com.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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