HSP27 Antibody (6H11) [Biotin]

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-25149B

Submit a review

Product Datasheet / COA / SDS

View Available Conjugates View Available Formulations

Conjugate

Catalog #

Alexa Fluor 350

NBP2-25149AF350

Alexa Fluor 405

NBP2-25149AF405

Alexa Fluor 488

NBP2-25149AF488

Alexa Fluor 532

NBP2-25149AF532

Alexa Fluor 594

NBP2-25149AF594

Alexa Fluor 647

NBP2-25149AF647

Alexa Fluor 700

NBP2-25149AF700

Alexa Fluor 750

NBP2-25149AF750

Alexa Fluor Plus 405

NBP2-25149AFP405

Alexa Fluor Plus 488

NBP2-25149AFP488

Alexa Fluor Plus 555

NBP2-25149AFP555

Alexa Fluor Plus 594

NBP2-25149AFP594

Alexa Fluor Plus 647

NBP2-25149AFP647

Alexa Fluor Plus 680

NBP2-25149AFP680

Allophycocyanin

NBP2-25149APC

CoraFluor 1

NBP2-25149CL1

DyLight 350

NBP2-25149UV

DyLight 405

NBP2-25149V

DyLight 488

NBP2-25149G

DyLight 550

NBP2-25149R

DyLight 594

NBP2-25149DL594

DyLight 650

NBP2-25149C

DyLight 680

NBP2-25149FR

DyLight 755

NBP2-25149IR

FITC

NBP2-25149F

HRP

NBP2-25149H

Janelia Fluor 525

NBP2-25149JF525

Janelia Fluor 549

NBP2-25149JF549

Janelia Fluor 646

NBP2-25149JF646

Janelia Fluor 669

NBP2-25149JF669

mFluor Violet 450 SE

NBP2-25149MFV450

mFluor Violet 500 SE

NBP2-25149MFV500

mFluor Violet 610 SE

NBP2-25149MFV610

PE

NBP2-25149PE

PerCP

NBP2-25149PCP

Forumulation

Catalog #

BSA Free

NBP2-25149

Product Specifications
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Human, Mouse (Negative), Rat (Negative)

Applications

Knockout Validated, Immunohistochemistry, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, CyTOF-ready

Label

Biotin

Antibody Source

Monoclonal Mouse IgG₁ Clone # 6H11

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all HSP27 Antibodies »

Product Specifications

Immunogen

Recombinant full length human HSP27 expressed in and purified from E. coli. [UniProt# P04792]

Reactivity Notes

Unreactive on Rodent.

Localization

Cytoplasm. Nucleus. Cytoplasm > cytoskeleton > spindle.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁

Description

This conjugate is made on demand and is not held in inventory. Each order represents a unique lot. All conjugates are generated using validated conjugation protocols and meet strict degree of labeling (F/P) specifications; additional information is available upon request. The antibody concentration is provided on the individual product label.

For specialized conjugation needs, like large quantities, specific concentrations, and defined F/P ratios, bulk and custom antibody conjugation services are available.

Applications for HSP27 Antibody (6H11) [Biotin]

Application

Recommended Usage

CyTOF-ready

Optimal dilutions of this antibody should be experimentally determined.

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Knockout Validated

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Application Notes

Recommended applications are based on validated applications from the unconjugated base product NBP2-25149. This conjugated antibody is not kept in inventory and is made to order using validated conjugation protocols.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: HSP27

Hsp25 is the mouse homologue of the human Hsp27 protein, a member of the small heat shock protein family comprised of a diverse group of proteins from approx. 15 to >30kDa(1). The basic structure of most sHsps is a homologous and highly conserved amino acid sequence, with an alpha-crystallin-domain at the C-terminus and the WD/EPF domain at the less conserved N-terminus. This N-terminus is essential for the development of high molecular oligomers (2, 3). Hsp27-oligomers consist of stable dimers formed by as many as 8-40 Hsp27 protein monomers (4). The oligomerization status is connected with the chaperone activity: aggregates of large oligomers have high chaperone activity, whereas dimers have no chaperone activity (5). HSP27 is localized to the cytoplasm of unstressed cells but can redistribute to the nucleus in response to stress, where it may function to stabilize DNA and/or the nuclear membrane. It can be rapidly phosphorylated in response to physiological stimuli relevant to the cell type examined. Thus, hsp27 has been suggested to be an important intermediate in second messenger mediated signaling pathways (6). Other functions include chaperone activity (as mentioned above), thermotolerance in vivo, inhibition of apoptosis, and signal transduction. Specifically, in vitro, it acts as an ATP-independent chaperone by inhibiting protein aggregation and by stabilizing partially denatured proteins, which ensures refolding of the HSP70 complex. Hsp27 is also involved in the apoptotic signaling pathway because it interferes with the activation of cytochrome c/Apaf-1/dATP complex, thereby inhibiting the activation of procaspase-9. It is also hypothesized that hsp27 may serve some role in cross-bridge formation between actin and myosin (7). And finally, Hsp27 is also thought to be involved in the process of cell differentiation. The up-regulation of Hsp27 correlates with the rate of phosphorylation and with an increase of large oligomers. It is possible that Hsp27 may play a crucial role in termination of growth (8).

Long Name

Heat Shock Protein 27

Alternate Names

HSP25, HSPB1

Gene Symbol

HSPB1

Additional HSP27 Products

Product Documents for HSP27 Antibody (6H11) [Biotin]

Product Datasheets

Download Product Datasheets

COA

SDS

Product Specific Notices for HSP27 Antibody (6H11) [Biotin]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations
Reviews