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HPV18 E6 Antibody (HPV18/1297)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-07521

Novus Biologicals, part of Bio-Techne
Catalog #
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NBP3-07521-100ug
NBP3-07521-20ug

Key Product Details

Species Reactivity

Virus

Applications

Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # HPV18/1297

Concentration

0.2 mg/ml

Product Specifications

Immunogen

HPV18 E6-beta-galactosidase fusion protein

Reactivity Notes

Type 18 of Human Papilloma Virus (HPV-18)

Localization

Nuclear

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Theoretical MW

16 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Description

200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 10 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-08845)

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.

Scientific Data Images for HPV18 E6 Antibody (HPV18/1297)

Immunohistochemistry-Paraffin: HPV18 E6 Antibody (HPV18/1297) [NBP3-07521]

Immunohistochemistry-Paraffin: HPV18 E6 Antibody (HPV18/1297) [NBP3-07521]

Immunohistochemistry-Paraffin: HPV18 E6 Antibody (HPV18/1297) [NBP3-07521] - Formalin-fixed, paraffin-embedded human Cervix stained with HPV18 E6 Mouse Monoclonal Antibody (HPV18/1297).
Immunohistochemistry-Paraffin: HPV18 E6 Antibody (HPV18/1297) [NBP3-07521]

Immunohistochemistry-Paraffin: HPV18 E6 Antibody (HPV18/1297) [NBP3-07521]

Immunohistochemistry-Paraffin: HPV18 E6 Antibody (HPV18/1297) [NBP3-07521] - Formalin-fixed, paraffin-embedded human Cervix stained with HPV18 E6 Mouse Monoclonal Antibody (HPV18/1297).

Applications for HPV18 E6 Antibody (HPV18/1297)

Application
Recommended Usage

Immunohistochemistry-Paraffin

1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10 mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C.

Background: HPV18 E6

This protein is a member of the keratin family. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. The type I cytokeratins consist of acidic proteins which are arranged in pairs of heterotypic keratin chains. Unlike its related family members, this smallest known acidic cytokeratin is not paired with a basic cytokeratin in epithelial cells. It is specifically expressed in the periderm, the transiently superficial layer that envelopes the developing epidermis. Keratin 19 is not expressed in hepatocytes, therefore, antibody to keratin 19 is useful in the identification of liver metastasis. The degree of keratin 19 positivity in breast cancer distinguishes malignant from benign tumours. Keratin 19 is often co-expressed with keratin 7.

Alternate Names

E6, HpV18gp1, Human papillomavirus type 18 E6, Protein E6

Additional HPV18 E6 Products

Product Documents for HPV18 E6 Antibody (HPV18/1297)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for HPV18 E6 Antibody (HPV18/1297)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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