HPV16 Antibody (HPV16L1/1058) - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-11542
Conjugate
Catalog #
Key Product Details
Species Reactivity
Virus
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2a Kappa Clone # HPV16L1/1058
Format
Azide and BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
Human HPV16, major capsid protein L1
Reactivity Notes
Type 16 of Human Papilloma Virus (HPV-16).
Localization
Nuclear
Specificity
Reacts with a protein of 57kDa, identified as the L1 protein of human papilloma virus type 16 (HPV-16). It is the major capsid protein of HPV-16. Infection with specific types of HPV has been associated with an increased risk of developing cervical neoplasia. HPV types 6 and 11 have been associated with relatively benign diseases such as genital warts but types 16 and 18 are strongly associated with cervical, vaginal, and vulvar malignancies. The antibody reacts very strongly with formalin-fixed, paraffin-embedded tissues containing HPV-16 or -33; very weak reactions were occasionally observed with biopsy specimens or smears containing HPV-6 or HPV-11. It cross-reacts with HPV37.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2a Kappa
Theoretical MW
57 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
1.0 mg/ml of antibody purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS WITHOUT BSA & azide. Also available at 200 ug/ml WITH BSA & azide (NBP2-45208).
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C.
Scientific Data Images for HPV16 Antibody (HPV16L1/1058) - Azide and BSA Free
Immunohistochemistry-Paraffin: HPV16 Antibody (HPV16L1/1058) - Azide and BSA Free [NBP3-11542] -
Immunohistochemistry-Paraffin: HPV16 Antibody (HPV16L1/1058) - Azide and BSA Free [NBP3-11542] - Human Cervix stained with HPV-16 Monoclonal Antibody (HPV16L1/1058)Applications for HPV16 Antibody (HPV16L1/1058) - Azide and BSA Free
Application
Recommended Usage
Immunohistochemistry-Paraffin
0.5 - 1.0 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Optimal dilution for a specific application should be determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Background: HPV16
Alternate Names
Human Papilloma Virus Type 16
Additional HPV16 Products
Product Documents for HPV16 Antibody (HPV16L1/1058) - Azide and BSA Free
Product Specific Notices for HPV16 Antibody (HPV16L1/1058) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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