HPa3 Antibody (HIC3-2D12) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-18520

Novus Biologicals, part of Bio-Techne
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NBP3-18520
NBP3-18520SS
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Flow Cytometry

Cited:

Flow Cytometry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgM Clone # HIC3-2D12

Format

BSA Free

Concentration

2 mg/ml

View all HPa3 Antibodies »

Product Specifications

Immunogen

Human pancreatic islets enriched in non-beta endocine cells.

Specificity

This HPa3 antibody does not label beta cells, labels delta cells dimly, and brightly labels alpha, gamma, and epsilon cells in human pancreatic islets.

Clonality

Monoclonal

Host

Mouse

Isotype

IgM

Scientific Data Images for HPa3 Antibody (HIC3-2D12) - BSA Free

Immunohistochemistry-Paraffin: HPa3 Antibody (HIC3-2D12) [NBP3-18520]

Immunohistochemistry-Paraffin: HPa3 Antibody (HIC3-2D12) [NBP3-18520]

Immunohistochemistry-Paraffin: HPa3 Antibody (HIC3-2D12) [NBP3-18520] - Formalin Fixed Paraffin Embedded (FFPE) human pancreatic tissue sections were prepared using heat-induced epitope retrieval (HIER). Immunostaining was performed using a 1:50 dilution of Cat. No. MABS1998, Anti-HPa3, clone HIC3-2D12. Reactivity was detected using an Rabbit Anti-Mouse and DAB. Cytoplasmic/membranous staining in islets of human pancreas (Left image). membranous staining in intercalated ducts (Middle image), as well as intralobular ducts of human pancreas (Right image) tissues was observed.

Applications for HPa3 Antibody (HIC3-2D12) - BSA Free

Application
Recommended Usage

Flow Cytometry

reported in scientific literature (PMID 27399229)

Immunohistochemistry

1:50

Immunohistochemistry-Paraffin

1:50. Use reported in scientific literature (PMID 27399229)

Formulation, Preparation, and Storage

Purification

Ion exchange chromatography

Formulation

PBS

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

2 mg/ml

Stability & Storage

Store at 4°C.

Background: HPa3

Stem cells offer a therapeutic promise for many diseases. In the case of diabetes, stem cells offer the promise that beta cells may be generated ex vivo for the possible transplantation and cure of Type I diabetes (Couri CE, et al.). For this to happen, stem cells will need to be isolated and expanded, they will need to be differentiated toward the beta cell lineage, and the differentiated cell progeny used for transplantation may need to be isolated from complex cell mixtures following differentiation. Appropriate markers targeting cell surface molecules on developing and mature pancreatic cells will be required for such research.

Additional HPa3 Products

Product Documents for HPa3 Antibody (HIC3-2D12) - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for HPa3 Antibody (HIC3-2D12) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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