HP1 alpha Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NB110-40623
![Western Blot: HP1 alpha Antibody [NB110-40623]](https://resources.bio-techne.com/images/products/HP1-alpha-Antibody-Western-Blot-NB110-40623-img0002.jpg "Western Blot: HP1 alpha Antibody [NB110-40623]")
Scientific images may be resized, cropped, or adjusted for brightness or contrast for presentation purposes. These updates are limited to presentation and do not affect the underlying scientific interpretation of the data. Where available, additional source data may be provided upon request.
Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human, Mouse
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunoprecipitation, Chromatin Immunoprecipitation (ChIP)
Cited:
Immunoprecipitation, Chemotaxis
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
1.0 mg/ml
Product Specifications
Immunogen
The immunogen recognized by this antibody maps to a region between residue 150 and the C-terminus (residue 191) of human Chromobox Homolog 5 using the numbering given in entry NP_036249.1 (GeneID 23468).
Marker
Heterochromatin Marker
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for HP1 alpha Antibody - BSA Free
Western Blot: HP1 alpha Antibody [NB110-40623]
Western Blot: HP1 alpha Antibody [NB110-40623] - Whole cell lysate (15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. NB110-40623 used for WB at 0.1 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 3 mcg/mg lysate (B).![Immunohistochemistry-Paraffin: HP1 alpha Antibody [NB110-40623]](https://resources.bio-techne.com/images/products/HP1-alpha-Antibody-Immunohistochemistry-NB110-40623-img0015.jpg "Immunohistochemistry-Paraffin: HP1 alpha Antibody [NB110-40623]")
Immunohistochemistry-Paraffin: HP1 alpha Antibody [NB110-40623]
Immunohistochemistry-Paraffin: HP1 alpha Antibody [NB110-40623] - Section of human prostate carcinoma. Antibody: Affinity purified rabbit anti-CBX5 used at a dilution of 1:5,000 (0.2ug/ml). Detection: DABApplications for HP1 alpha Antibody - BSA Free
Application
Recommended Usage
Chromatin Immunoprecipitation (ChIP)
1:10-1:500
Immunohistochemistry
1:200-1:10000
Immunohistochemistry-Paraffin
1:200-1:10000
Immunoprecipitation
2-5 ug/mg lysate
Western Blot
1:1000-1:10000
Application Notes
Chip, IP reactivity reported in (PMID: 23045645). Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Format
BSA Free
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: HP1 alpha
Alternate Names
Antigen p25, chromobox homolog 5, chromobox homolog 5 (Drosophila HP1 alpha), chromobox homolog 5 (HP1 alpha homolog, Drosophila), chromobox protein homolog 5, Heterochromatin protein 1 homolog alpha, heterochromatin protein 1-alpha, HP1, HP1 alpha, HP1 alpha homolog, HP1A, HP1-ALPHA, HP1Hs alpha, HP1Hs-alpha
Entrez Gene IDs
23468 (Human)
Gene Symbol
CBX5
UniProt
Additional HP1 alpha Products
Product Documents for HP1 alpha Antibody - BSA Free
Product Specific Notices for HP1 alpha Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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