HOXA11 Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-80228
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human, Mouse
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
0.5 mg/ml
Product Specifications
Immunogen
Synthetic peptide directed towards the C terminal of human HOXA11. Peptide sequence KERRRRPESSSSPESSSGHTEDKAGGSGGQRTRKKRCPYTKYQIRELERE. The peptide sequence for this immunogen was taken from within the described region.
Reactivity Notes
Human reactivity reported in scientific literature (PMID: 31665361).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
34 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
The addition of 50% glycerol is optional for those storing this antibody at -20C and not aliquoting smaller units. However, please note that glycerol may interrupt some downstream antibody applications and should be added with caution.
Scientific Data Images for HOXA11 Antibody - BSA Free
Western Blot: HOXA11 Antibody [NBP1-80228]
Western Blot: HOXA11 Antibody [NBP1-80228] - Titration: 0.2-1 ug/ml, Positive Control: Human Uterus.Immunohistochemistry-Paraffin: HOXA11 Antibody [NBP1-80228]
Immunohistochemistry-Paraffin: HOXA11 Antibody [NBP1-80228] - Human Uterus Tissue, 4-8ug/ml.Western Blot: HOXA11 Antibody - BSA Free [NBP1-80228] -
BM transplantation from WT donors leads to decidualization reaction in Hoxa11-null mice.(A-H) Sections of E5.5 implantation sites from WTWT BMT, KOWT BMT, and KOKO BMT stained with HE (A and B), CD31 (C and D), PR (E and F), and PCNA (G and H). B, D, F, and H are higher magnification photomicrographs of A, C, E, and G, respectively. Black arrows point to embryos. (J, K and L) Quantification of pecent PR-positive cells (J), percent PCNA-positive cells (K), and mean blood vessel luminal area (μm2) in the endometrium (n = 3–4 mice/group). (I) Sections of E5.5 implantation sites from WTWT BMT, KOWT BMT, and KOKO BMT co-stained with Hoxa11 (green), CD45 (red), and counterstained with DAPI (blue). Arrows point to Hoxa11-expressing cells. (M) Relative uterine mRNA expression of HoxA11 and implantation-related genes Prl8a2, Lif, and Msx1 normalized to GAPDH in WTWT BMT versus KOWT BMT versus KOKO BMT on E5.5 (n = 4–5 mice/group). (N) Uterine Hoxa11 protein expression in KOWT BMT versus KOKO BMT on E5.5. Bar graphs represent mean +/- SEM. p-values are noted on the graphs. Scale bars, 100 μm (A, C, E, G), 50 μm (B, D, F, H). Underlying data are available in S1 Data. BM, bone marrow; BMT, BM transplant; GAPDH, glyceraldehyde 3-phosphate dehydrogenase; HE, hematoxylin–eosin; Hoxa11, Homeobox a11; KO, knockout; PCNA, proliferating cell nuclear antigen; PR, progesterone receptor; WT, wild-type. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/31513564), licensed under a CC-BY license. Not internally tested by Novus Biologicals.Applications for HOXA11 Antibody - BSA Free
Application
Recommended Usage
Immunohistochemistry
1:10-1:500
Immunohistochemistry-Paraffin
1:10-1:500
Western Blot
1.0 ug/ml
Reviewed Applications
Read 1 review rated 5 using NBP1-80228 in the following applications:
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS, 2% Sucrose
Format
BSA Free
Preservative
0.09% Sodium Azide
Concentration
0.5 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: HOXA11
Alternate Names
homeo box A11, homeobox A11, Homeobox protein Hox-1I, homeobox protein HOXA11, homeobox protein Hox-A11, HOX1, HOX1Ihomeo box 1I
Gene Symbol
HOXA11
UniProt
Additional HOXA11 Products
Product Documents for HOXA11 Antibody - BSA Free
Product Specific Notices for HOXA11 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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