Host Cell Factor 1/HCFC1 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NB100-68210
Key Product Details
Validated by
Independent Antibodies
Species Reactivity
Validated:
Human, Mouse
Cited:
Mouse
Predicted:
Golden Syrian Hamster (100%). Backed by our 100% Guarantee.
Applications
Validated:
Western Blot, Simple Western, Immunoprecipitation
Cited:
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
0.2 mg/ml
Product Specifications
Immunogen
The immunogen recognized by this antibody maps to a region between residue 1985 and 2035 of human host cell factor C1 (VP16-accessory protein) using the numbering given in entry NP_005325.2 (GeneID 3054).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
Novus Biologicals Rabbit Host Cell Factor 1/HCFC1 Antibody (NB100-68210) is a polyclonal antibody validated for use in WB, Simple Western and IP. Anti-Host Cell Factor 1/HCFC1 Antibody: Cited in 2 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for Host Cell Factor 1/HCFC1 Antibody
Simple Western: Host Cell Factor 1/HCFC1 Antibody [NB100-68210]
Host-Cell-Factor-1-HCFC1-Antibody-Simple-Western-NB100-68210-img0005.jpgWestern Blot: Host Cell Factor 1/HCFC1 Antibody [NB100-68210] -
Western Blot: Host Cell Factor 1/HCFC1 Antibody [NB100-68210] - Transcriptional activation by E2F4 in mouse ES cells is mediated by chromatin modifiers. a Schematic representation of the affinity purification-mass spectrometry approach to identify the E2F4 interactome. b Silver stain of eluted fractions from RPE cells and mouse ES cells . Slices of gel were subjected to mass spectrometry, & the indicated protein bands were identified by analyzing the proteins enriched in each slice. c GO terms for cellular components enriched in the list of mouse ES cell-specific candidate interactors. d Validation of interactions between GFP-E2F4 & DP-1, HCFC1, YEATS2, LIN54, & LIN9, by co-immunoprecipitation followed by immunoassay, in mouse ES cells & human RPE cells. Transcriptional activators (HCFC1 & YEATS2) preferentially bind to E2F4 in mouse ES cells while members of the DREAM repressor complex (LIN54 & LIN9) bind preferentially to E2F4 in RPE cells. Molecular weights (kDa) are indicated on the left side (one experiment shown of at least two experiments) Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/31270324), licensed under a CC-BY license. Not internally tested by Novus Biologicals.Applications for Host Cell Factor 1/HCFC1 Antibody
Application
Recommended Usage
Immunoprecipitation
2-5 ug/mg lysate
Western Blot
1:2000-1:10000
Application Notes
See Simple Western Antibody Database for Simple Western validation: Tested in Mouse ES cells and Human RPE cells, separated by Size
Reviewed Applications
Read 1 review rated 5 using NB100-68210 in the following applications:
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
TBS and 0.1% BSA
Preservative
0.09% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: Host Cell Factor 1/HCFC1
Additional Host Cell Factor 1/HCFC1 Products
Product Documents for Host Cell Factor 1/HCFC1 Antibody
Product Specific Notices for Host Cell Factor 1/HCFC1 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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