Hormone-sensitive Lipase/HSL [p Ser563] Antibody - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-05457
Key Product Details
Validated by
Biological Validation
Species Reactivity
Validated:
Human, Mouse, Rat
Cited:
Mouse
Applications
Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
Azide and BSA Free
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
A synthetic phosphorylated peptide around S563 of rat Hormone-sensitive Lipase/HSL (XP_006228456.1). RRSVS
Reactivity Notes
Mouse reactivity reported from a verified customer review.
Modification
p Ser563
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for Hormone-sensitive Lipase/HSL [p Ser563] Antibody - Azide and BSA Free
Western Blot: Hormone-sensitive Lipase/HSL [p Ser563] Antibody [NBP3-05457] - Western blot analysis of extracts of various cell lines, using Hormone-sensitive Lipase/HSL antibody (NBP3-05457) at 1:1000 dilution.3T3L1 cells were treated by lambda-PP mixed solution (1ul) at 30C for 30 minutes.H9C2 cells were treated with Isoproterenol (10 uM for 20 min) at 37C. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.
![Hormone-sensitive Lipase/HSL [p Ser563] Antibody - Azide and BSA Free](https://resources.bio-techne.com/images/products/nbp3-05457_rabbit-polyclonal-hormone-sensitive-lipase-hsl-p-ser563-antibody-52202516334111.jpg "Western Blot: Hormone-sensitive Lipase/HSL [p Ser563] Antibody - Azide and BSA Free [Hormone-sensitive Lipase/HSL] -")
Western Blot: Hormone-sensitive Lipase/HSL [p Ser563] Antibody - Azide and BSA Free [Hormone-sensitive Lipase/HSL] -
Western Blot: Hormone-sensitive Lipase/HSL [p Ser563] Antibody - Azide and BSA Free [Hormone-sensitive Lipase/HSL] - Western blot analysis of lysates from Mouse brown fat using Hormone-sensitive Lipase/HSL Rabbit pAb at 1:5000 dilution incubated overnight at 4C. Mouse brown fat were treated by lambda-PP mixed solution (1 ul) at 30C for 30 minutes.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 30 ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit .
Exposure time: 1s.
Applications for Hormone-sensitive Lipase/HSL [p Ser563] Antibody - Azide and BSA Free
Application
Recommended Usage
Western Blot
1:500 - 1:1000
Application Notes
Hormone-sensitive Lipase/HSL [p Ser563] antibody validated for WB from a verified customer review.
Reviewed Applications
Read 1 review rated 5 using NBP3-05457 in the following applications:
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.3), 50% glycerol
Format
Azide and BSA Free
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Hormone-sensitive Lipase/HSL
HSL is the major enzyme responsible for the mobilization of free fatty acids from adopise tissue, and HSL antibodies are useful tools for lipid and metabolism research.
Alternate Names
Hormonesensitive Lipase, HSL, LHS, LIPE
Gene Symbol
LIPE
Additional Hormone-sensitive Lipase/HSL Products
Product Documents for Hormone-sensitive Lipase/HSL [p Ser563] Antibody - Azide and BSA Free
Product Specific Notices for Hormone-sensitive Lipase/HSL [p Ser563] Antibody - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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⚠ WARNING: This product can expose you to chemicals including Methotrexate, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov