HMGCS1 Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-36554
![Western Blot: HMGCS1 Antibody [NBP2-36554]](https://resources.bio-techne.com/images/products/HMGCS1-Antibody-Western-Blot-NBP2-36554-img0001.jpg "Western Blot: HMGCS1 Antibody [NBP2-36554]")
Conjugate
Catalog #
Key Product Details
Validated by
Biological Validation
Species Reactivity
Validated:
Human, Mouse
Cited:
Human
Predicted:
Orangutan (100%). Backed by our 100% Guarantee.
Applications
Validated:
Western Blot, Simple Western, Immunoprecipitation
Cited:
Simple Western
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
1.0 mg/ml
Product Specifications
Immunogen
The immunogen recognized by the antibody maps to a region between residue 470 to 520 of human 3-hydroxy-3-methylglutaryl-CoA Synthase 1 using the numbering given in entry NP_001091742.1.
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for HMGCS1 Antibody - BSA Free
Western Blot: HMGCS1 Antibody [NBP2-36554]
Western Blot: HMGCS1 Antibody [NBP2-36554] - Samples: Whole cell lysate (50 ug) from HeLa, 293T, Jurkat, mouse TCMK-1, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-HMGCS1 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.![Immunoprecipitation: HMGCS1 Antibody [NBP2-36554]](https://resources.bio-techne.com/images/products/HMGCS1-Antibody-Immunoprecipitation-NBP2-36554-img0002.jpg "Immunoprecipitation: HMGCS1 Antibody [NBP2-36554]")
Immunoprecipitation: HMGCS1 Antibody [NBP2-36554]
Immunoprecipitation: HMGCS1 Antibody [NBP2-36554] - Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. For blotting immunoprecipitated HMGCS1, it was used at 0.4 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.Applications for HMGCS1 Antibody - BSA Free
Application
Recommended Usage
Immunoprecipitation
2 - 10 ug/mg
Simple Western
1:50
Western Blot
1:2000 - 1:10000
Application Notes
Antibody concentration was determined by extinction coefficient: absorbance at 280 nm of 1.4 equals 1.0 mg of IgG.
Validation by IP/Western Blot was performed using a 4-20% SDS-PAGE gel. See Simple Western Antibody Database for Simple Western validation: Tested in Arteries, separated by Size, antibody dilution of 1:50
Validation by IP/Western Blot was performed using a 4-20% SDS-PAGE gel. See Simple Western Antibody Database for Simple Western validation: Tested in Arteries, separated by Size, antibody dilution of 1:50
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Format
BSA Free
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: HMGCS1
Alternate Names
3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) synthase, 3-hydroxy-3-methylglutaryl coenzyme A synthase, 3-hydroxy-3-methylglutaryl-CoA synthase 1 (soluble), 3-hydroxy-3-methylglutaryl-Coenzyme A synthase 1 (soluble), EC 2.3.3.10, HMG-CoA synthase, HMGCS, hydroxymethylglutaryl-CoA synthase, cytoplasmic, MGC90332
Gene Symbol
HMGCS1
UniProt
Additional HMGCS1 Products
Product Documents for HMGCS1 Antibody - BSA Free
Product Specific Notices for HMGCS1 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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