HLA G Antibody (HLAG/8344R)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-20480
Recombinant Monoclonal Antibody
Scientific images may be resized, cropped, or adjusted for brightness or contrast for presentation purposes. These updates are limited to presentation and do not affect the underlying scientific interpretation of the data. Where available, additional source data may be provided upon request.
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Kappa Clone # HLAG/8344R
Concentration
0.2 mg/ml
Product Specifications
Immunogen
Purified His-tagged HLA G protein.
Localization
Cell surface.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG Kappa
Description
Positive Controls: Raji cells. Expressed in trophoblasts.
Antibody with azide - store at 2 to 8C. Non-hazardous. No MSDS required.
Antibody with azide - store at 2 to 8C. Non-hazardous. No MSDS required.
Scientific Data Images for HLA G Antibody (HLAG/8344R)
HLA G Antibody (HLAG/8344R)
Formalin-fixed, paraffin-embedded human placenta stained with HLA G antibody (HLAG/8344R). Inset: PBS instead of primary antibody; secondary only negative control.
HLA G Antibody (HLAG/8344R)
Formalin-fixed, paraffin-embedded human tonsil stained with HLA G antibody (HLAG/8344R). Inset: PBS instead of primary antibody; secondary only negative control.
HLA G Antibody (HLAG/8344R)
Formalin-fixed, paraffin-embedded human bladder carcinoma stained with HLA G antibody (HLAG/8344R). HIER: Tris/EDTA, pH9.0, 45min. 2: HRP-polymer, 30min. DAB, 5min.Applications for HLA G Antibody (HLAG/8344R)
Application
Recommended Usage
Immunohistochemistry-Paraffin
1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes. at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.
Optimal dilution for a specific application should be determined.
Optimal dilution for a specific application should be determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10 mM PBS with 0.05% BSA
Preservative
0.05% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: HLA-G
Discovered in cytotrophoblasts, HLA-G is involved in fetal maternal immune tolerance and some studies have linked its downregulation with severe preeclampsia (3). HLA-G mediated immune suppression works by impeding cell proliferation, differentiation, cytotoxicity, cytokine secretion and chemotaxis; and activation of regulatory cells and MDSCs or M2 type macrophage. HLA-G is constitutively expressed in immune-privileged sites such as pancreatic islets, mesenchymal stem cells (MSCs) and the cornea. Upregulation of HLA-G occurs in pathological states including cancer, allo-transplantations, viral infections, autoimmune and inflammatory diseases (4). In cancer, HLA-G expression is induced by hypoxia and has been correlated with advanced tumor stage, tumor metastasis, and poor therapeutic response and survival. HLA-G is an attractive tumor associated-antigen (TAA) for immunotherapy and is considered a major immune checkpoint (ICP).
References
1. Loustau, M., Anna, F., Drean, R., Lecomte, M., Langlade-Demoyen, P., & Caumartin, J. (2020). HLA-G Neo-Expression on Tumors. Front Immunol, 11:1685. PMID: 32922387
2. Lin A, Yan WH. (2018) Heterogeneity of HLA-G Expression in Cancers: Facing the Challenges. Front Immunol. 9:2164. PMID: 30319626
3. Djurisic S, Hviid TV. (2014) HLA Class Ib Molecules and Immune Cells in Pregnancy and Preeclampsia. Front Immunol. 5:652. PMID: 25566263
4. Lila N, Rouas-Freiss N, Dausset J, Carpentier A, Carosella ED. (2001) Soluble HLA-G protein secreted by allo-specific CD4+ T cells suppresses the allo-proliferative response: a CD4+ T cell regulatory mechanism. Proc Natl Acad Sci U S A. 98(21):12150-12155. PMID: 11572934
Long Name
Major Histocompatibility Complex, Class I, G
Alternate Names
HLA G, HLA-6.0, HLAG, MHC Class I Antigen G, MHC-G
Gene Symbol
HLA-G
Additional HLA-G Products
Product Documents for HLA G Antibody (HLAG/8344R)
Product Specific Notices for HLA G Antibody (HLAG/8344R)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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