HLA ABC Antibody (YTH862.2) - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NB100-65411
![Flow Cytometry: HLA ABC Antibody (YTH862.2) - BSA Free [NB100-65411]](https://resources.bio-techne.com/images/products/HLA-ABC-Antibody-YTH862-2-Flow-Cytometry-NB100-65411-img0001.jpg "Flow Cytometry: HLA ABC Antibody (YTH862.2) - BSA Free [NB100-65411]")
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Conjugate
Catalog #
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Immunohistochemistry-Frozen, Flow Cytometry, Immunoprecipitation, Functional
Label
Unconjugated
Antibody Source
Monoclonal Rat IgG2B Clone # YTH862.2
Format
BSA Free
Concentration
1.0 mg/ml
Product Specifications
Immunogen
PHA activated human peripheral blood lymphocytes
Specificity
NB100-65411 recognizes a monomorphic determinant on the human HLA Class I heavy chain. Epitope analysis has shown that this antibody binds in the alpha1 domain. MHC Class I is expressed by all nucleated cells. Recent studies have shown that this antibody may be able to induce apoptosis in activated but not resting T cells (1).
Clonality
Monoclonal
Host
Rat
Isotype
IgG2B
Scientific Data Images for HLA ABC Antibody (YTH862.2) - BSA Free
Flow Cytometry: HLA ABC Antibody (YTH862.2) - BSA Free [NB100-65411]
Flow Cytometry: HLA ABC Antibody (YTH862.2) [NB100-65411] - Staining of human peripheral blood lymphocytes.Applications for HLA ABC Antibody (YTH862.2) - BSA Free
Application
Recommended Usage
Flow Cytometry
1:50-1:100
Immunohistochemistry
1:10-1:500
Immunohistochemistry-Frozen
1:10-1:500
Immunoprecipitation
1:10-1:500
Application Notes
Flow Cytometry: Use 10 ul of the suggested working dilution to label 10^6 cells in 100 ul. This product contains sodium azide, removal by dialysis is recommended prior to use in functional assays.
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS
Format
BSA Free
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: HLA ABC
Alternate Names
A-10 alpha chain, FLJ26655, HLA class I histocompatibility antigen, A-1 alpha chain, HLA class I histocompatibility antigen, A-28 alpha chain, HLA class I histocompatibility antigen, A-9 alpha chain, HLAA, major histocompatibility complex, class I, A, MHC class I antigen A*1, MHC class I antigen A*11, MHC class I antigen A*80
Gene Symbol
HLA-A
Additional HLA ABC Products
Product Documents for HLA ABC Antibody (YTH862.2) - BSA Free
Product Specific Notices for HLA ABC Antibody (YTH862.2) - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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