HJURP Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-47287

Novus Biologicals, part of Bio-Techne

Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1.0 mg/ml

View all HJURP Antibodies »

Product Specifications

Immunogen

The immunogen for this product maps to a region between residue 650 and 700 of human Holliday Junction Recognition Protein using the numbering given in entry NP_060880.3 (GeneID 55355).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for HJURP Antibody - BSA Free

Western Blot: HJURP Antibody [NBP1-47287]

Western Blot: HJURP Antibody [NBP1-47287]

Western Blot: HJURP Antibody [NBP1-47287] - Detection of human HJURP by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and HEK293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-HJURP antibody NBP1-47287 used for WB at 0.1 ug/ml (A) and 1 ug/ml (B) and used for IP at 3 ug/mg lysate. HJURP was also immunoprecipitated by rabbit anti-HJURP antibody NBP1-472878, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).
HJURP Antibody [NBP1-47287]

HJURP Antibody [NBP1-47287]-Section of human lung carcinoma. Antibody: Affinity purified rabbit anti-HJURP used at a dilution of 1:5,000 (0.2µg/ml) Detection: DAB

HJURP Antibody [NBP1-47287]-Section of human lung carcinoma. Antibody: Affinity purified rabbit anti-HJURP used at a dilution of 1:5,000 (0.2µg/ml) Detection: DAB
HJURP Antibody [NBP1-47287]

HJURP Antibody [NBP1-47287]- Whole cell lysate (2 µg) from K-562, U2OS, HeLa, and MCF-7 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-HJURP antibody used for WB at 0.04 µg/ml. Detection:Chemiluminescence with an exposure time of 75 seconds.

HJURP Antibody [NBP1-47287]- Whole cell lysate (2 µg) from K-562, U2OS, HeLa, and MCF-7 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-HJURP antibody used for WB at 0.04 µg/ml. Detection:Chemiluminescence with an exposure time of 75 seconds.

Applications for HJURP Antibody - BSA Free

Application
Recommended Usage

Immunohistochemistry

1:1000 - 1:5000

Immunohistochemistry-Paraffin

1:1000 - 1:5000

Immunoprecipitation

2 -10 ug/mg lysate

Western Blot

1:2000 - 1:10000
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: HJURP

Centromeric protein that plays a central role in the incorporation and maintenance of histone H3-like variant CENPA at centromeres. Acts as a specific chaperone for CENPA and is required for the incorporation of newly synthesized CENPA molecules into nucleosomes at replicated centromeres. Directly binds Holliday junctions

Alternate Names

FAKTS14-3-3-associated AKT substrate, fetal liver expressing gene 1, Fetal liver-expressing gene 1 protein, FLEG1, hFLEG1, Holliday junction recognition protein, Up-regulated in lung cancer 9, URLC9DKFZp762E1312

Entrez Gene IDs

55355 (Human)

Gene Symbol

HJURP

Additional HJURP Products

Product Documents for HJURP Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for HJURP Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.