Histone H4 [ac Lys8] Antibody - BSA Free

Western Blot: Histone H4 [ac Lys8] Antibody [NBP2-59260]

Western Blot: Histone H4 [ac Lys8] Antibody [NBP2-59260] - Histone extracts of HeLa cells (15 ug) were analysed by Western blot using the antibody directed against H4K8ac diluted 1:200 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.

Immunocytochemistry/ Immunofluorescence: Histone H4 [ac Lys8] Antibody [NBP2-59260]

Immunocytochemistry/Immunofluorescence: Histone H4 [ac Lys8] Antibody [NBP2-59260] - Mouse NIH3T3 cells were stained with the antibody against H4K8ac and with DAPI. Cells were fixed with 4% formaldehyde for 10' and blocked with PBS/TX-100 containing 5% normal goat serum and 1% BSA. The cells were immunofluorescently labelled with the H4K8ac antibody (left) diluted 1:500 in blocking solution followed by an anti-rabbit antibody conjugated to Alexa Fluor 488. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.

Chromatin Immunoprecipitation: Histone H4 [ac Lys8] Antibody [NBP2-59260] - ChIP assays were performed using human HeLa cells, the antibody directed against H4K8ac and optimized PCR primer sets for qPCR. ChIP was performed with a LowCell l# ChIP kit on sheared chromatin from 10,000 cells. A titration of the antibody consisting of 1, 2, 5, and 10 ug per ChIP experiment was analysed. IgG (1 ug/IP) was used as negative IP control. QPCR was performed with primers for the c-fos promoter and for the Sat2 satellite repeat region. Figure shows the recovery, expressed as a % of input (the relative amount of immunoprecipitated DNA compared to input DNA after qPCR analysis).