Histone H3 Antibody (JE46-51) - K27M mutant

Chromatin Immunoprecipitation: Histone H3 Antibody (JE46-51) - K27M mutant [NBP3-32401]

Chromatin Immunoprecipitation: Histone H3 Antibody (JE46-51) - K27M mutant [NBP3-32401] - Chromatin immunoprecipitations were performed with cross-linked chromatin from HeLa cells and either Histone H3 (NBP3-32401) or Normal Rabbit IgG according to the ChIP protocol. The enriched DNA was quantified by real-time PCR using indicated primers. The amount of immunoprecipitated DNA in each sample is represented as signal relative to the total amount of input chromatin, which is equivalent to one.

Immunohistochemistry: Histone H3 Antibody (JE46-51) - K27M mutant [NBP3-32401]

Immunohistochemistry: Histone H3 Antibody (JE46-51) - K27M mutant [NBP3-32401] - Immunohistochemical analysis of paraffin-embedded 293T cells transfacted with Histone H3 / Histone H3 K27M mutant with Rabbit anti-Histone H3 antibody (NBP3-32401) at 1/1,000 dilution.

The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (NBP3-32401) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunocytochemistry/ Immunofluorescence: Histone H3 Antibody (JE46-51) - K27M mutant [NBP3-32401]

Immunocytochemistry/ Immunofluorescence: Histone H3 Antibody (JE46-51) - K27M mutant [NBP3-32401] - Immunocytochemistry analysis of HeLa cells transfacted with Histone H3 / Histone H3 labeling Histone H3 (K27M mutant ) with Rabbit anti-Histone H3 antibody (NBP3-32401) at 1/1,000 dilution.

Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Histone H3 antibody (NBP3-32401) at 1/1,000 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

Beta tubulin (red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594) was used as the secondary antibody at 1/1,000 dilution.