Histone H2A.Z Antibody (2X2I10)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16447
Recombinant Monoclonal Antibody
Key Product Details
Validated by
Knockout/Knockdown
Species Reactivity
Human, Mouse, Rat
Applications
Western Blot, Chromatin Immunoprecipitation (ChIP)
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 2X2I10 expressed in HEK293
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Histone H2A.Z (P0C0S5). MAGGKAGKDSGKAKTKAVSRSQRAGLQFPVGRIHRHLKSRTTSHGRVGATAAVYSAAILEYLTAEVLELAGNASKDLKVKRITPRHLQLAIRGDEELDSL
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Description
Novus Biologicals Rabbit Histone H2A.Z Antibody (2X2I10) (NBP3-16447) is a recombinant monoclonal antibody validated for use in WB and ChIP. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for Histone H2A.Z Antibody (2X2I10)
![Western Blot: Histone H2A.Z Antibody (2X2I10) [NBP3-16447]](https://resources.bio-techne.com/images/products/Histone-H2A-Z-Antibody-2X2I10-Western-Blot-NBP3-16447-img0001.jpg "Western Blot: Histone H2A.Z Antibody (2X2I10) [NBP3-16447]")
Western Blot: Histone H2A.Z Antibody (2X2I10) [NBP3-16447]
Western Blot: Histone H2A.Z Antibody (2X2I10) [NBP3-16447] - Western blot analysis of extracts of various cell lines, using Histone H2A.Z Rabbit mAb (NBP3-16447) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
Chromatin Immunoprecipitation: Histone H2A.Z Antibody (2X2I10) [NBP3-16447] -
Chromatin Immunoprecipitation: Histone H2A.Z Antibody (2X2I10) [NBP3-16447] - Chromatin immunoprecipitation analysis of extracts of HeLa cells, using Histone H2A.Z Rabbit mAb antibody and rabbit IgG.The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.
Western Blot: Histone H2A.Z Antibody (2X2I10) [NBP3-16447] -
Western Blot: Histone H2A.Z Antibody (2X2I10) [NBP3-16447] - Western blot analysis of lysates from wild type(WT) and Histone H2A.Z knockout (KO) 293T(KO) cells, using [KO Validated] Histone H2A.Z Rabbit mAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 1s.
Applications for Histone H2A.Z Antibody (2X2I10)
Application
Recommended Usage
Chromatin Immunoprecipitation (ChIP)
1:50-1:200
Western Blot
1:1000 - 1:5000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.3), 50% glycerol, 0.05% BSA
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Histone H2A.Z
Alternate Names
H2A histone family, member Z, H2A.Z, H2A/z, H2AZ histone, H2AZMGC117173, histone H2A.Z
Gene Symbol
H2AZ1
Additional Histone H2A.Z Products
Product Documents for Histone H2A.Z Antibody (2X2I10)
Product Specific Notices for Histone H2A.Z Antibody (2X2I10)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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