Histone H2AY/macroH2A.1 Antibody (CL5245) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-44248

Novus Biologicals, part of Bio-Techne
Catalog #
Availability
Size / Price
Qty
NBP3-44248
Print Quote
Bulk Order
Forumulation
Catalog #
BSA Free
NBP2-61155
View Product
100% Guarantee

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # CL5245

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all Histone H2AY Antibodies »

Product Specifications

Immunogen

This antibody was generated using a synthetic peptide of O75367, with the exact immunogen sequence remaining proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Description

Novus Biologicals Mouse Histone H2AY/macroH2A.1 Antibody (CL5245) - Azide and BSA Free (NBP2-61155) is a monoclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for Histone H2AY/macroH2A.1 Antibody (CL5245) - Azide and BSA Free

Histone H2AY/macroH2A.1 Antibody (CL5245) - Azide and BSA Free Immunocytochemistry/ Immunofluorescence: Histone H2AY/macroH2A.1 Antibody [NBP3-44248]

Immunocytochemistry/ Immunofluorescence: Histone H2AY/macroH2A.1 Antibody [NBP3-44248]

Staining of CACO-2 cells using the Anti-Histone H2AY/macroH2A.1 monoclonal antibody) .
Histone H2AY/macroH2A.1 Antibody (CL5245) - Azide and BSA Free Immunohistochemistry-Paraffin: Histone H2AY/macroH2A.1 Antibody [NBP3-44248]

Immunohistochemistry-Paraffin: Histone H2AY/macroH2A.1 Antibody [NBP3-44248]

Staining of human cerebellum shows strong nuclear immunoreactivity in neuronal and glial cells.
Histone H2AY/macroH2A.1 Antibody (CL5245) - Azide and BSA Free Immunohistochemistry-Paraffin: Histone H2AY/macroH2A.1 Antibody [NBP3-44248]

Immunohistochemistry-Paraffin: Histone H2AY/macroH2A.1 Antibody [NBP3-44248]

Staining of human fallopian tube shows strong nuclear positivity in glandular and stromal cells.
View 5 more images

Applications for Histone H2AY/macroH2A.1 Antibody (CL5245) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

2-10 ug/ml

Immunohistochemistry-Paraffin

1:2500 - 1:5000

Western Blot

1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. For Immunocytochemistry/ Immunofluorescence/IF, PFA/Triton X-100 is recommended for fixation/permeabilization.

Formulation, Preparation, and Storage

Purification

Protein A purified

Reconstitution

Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Formulation

Lyophilized from a 0.2 um filtered solution in PBS with Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Histone H2AY

macroH2A.1 (H2AFY) is 372 amino acids long, weighs approximately 40 kDa, and is part of a group of basic nuclear proteins called histones, which are responsible for nucleosome structure of the chromosomal fiber in eukaryotes. macroH2a.1 has two shorter isoforms meeasuring 371 and 369 amino acids long, both weighing approximately 39 kDa. Current studies are being done on several diseases and disorders including medulloblastoma, lupus erythematosus, Huntington's disease, immunodeficiency, melanoma, and malaria. macroH2A.1 has also been shown to have interactions with HIST1H4A, HIST1H4B, HIST1H4C, HIST1H4D, and HIST1H4E in pathways such as the chromatin regulation/ acetylation, systemic lupus erythematosus, and ATF-2 transcription factor pathways.

Long Name

H2A Histone Family, Member Y

Alternate Names

H2AF12M, H2AFJ, H2AFY, Histone MacroH2A1, Medulloblastoma Antigen MU-MB-50.205

Gene Symbol

MACROH2A1

Additional Histone H2AY Products

Product Documents for Histone H2AY/macroH2A.1 Antibody (CL5245) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Histone H2AY/macroH2A.1 Antibody (CL5245) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.