Histone H2AX Antibody (RM214)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-18534
Recombinant Monoclonal
![Western Blot: Histone H2AX Antibody (RM214) [NBP3-18534]](https://resources.bio-techne.com/images/products/Histone-H2AX-Antibody-RM214-Western-Blot-NBP3-18534-img0001.jpg "Western Blot: Histone H2AX Antibody (RM214) [NBP3-18534]")
Conjugate
Catalog #
Forumulation
Catalog #
Key Product Details
Species Reactivity
Vertebrate
Applications
ELISA, Immunocytochemistry/ Immunofluorescence, Multiplex Immunofluorescence, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # RM214 expressed in HEK293
Concentration
1 mg/ml
Product Specifications
Immunogen
A peptide corresponding to the C-terminus of human Histone H2AX
Specificity
This antibody reacts to Histone H2AX protein, independent of post-translational modifications. No cross reactivity with other histone proteins.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Description
Novus Biologicals Rabbit Histone H2AX Antibody (RM214) (NBP3-18534) is a recombinant monoclonal antibody validated for use in Multiplex Immunofluorescence, WB, ELISA and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for Histone H2AX Antibody (RM214)
Western Blot: Histone H2AX Antibody (RM214) [NBP3-18534]
Western Blot: Histone H2AX Antibody (RM214) [NBP3-18534] - Western Blot of A375, HEK293, HeLa and SK-MEL-2 whole cell lysates, using NBP3-18534 at 0.5 ug/mL, showed endogenous Histone H2AX in A375, HEK293, HeLa and SK-MEL-2 cells.
Immunocytochemistry/ Immunofluorescence: Histone H2AX Antibody (RM214) - Azide and BSA Free [NBP3-18534] -
Immunocytochemistry/ Immunofluorescence: Histone H2AX Antibody (RM214) - Azide and BSA Free [NBP3-18534] - Immunocytochemistry of HeLa cells, using Clone RM214 (red). Actin filaments have been labeled with fluorescein phalloidin (green).Applications for Histone H2AX Antibody (RM214)
Application
Recommended Usage
ELISA
0.2 ug/ml - 1 ug/ml
Immunocytochemistry/ Immunofluorescence
1 ug/mL - 2 ug/ml
Western Blot
0.5 ug/mL - 2 ug/ml
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
50% Glycerol/PBS, 1% BSA
Preservative
0.09% Sodium Azide
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C.
Background: Histone H2AX
Histone H2AX is one of a number of core histone proteins. In the cellular response to genotoxic insults, ATM and related protein kinases phosphorylate the carboxyl-terminal tail of the H2AX protein (gamma-H2AX). gamma-H2AX marks the site of damage and provides a nucleation site for the formation of damage response and repair complexes. BRCA1 carboxyl terminal (BRCT) domain-containing proteins are recruited to gamma-H2AX to create a protein scaffold for further assembly of signaling complexes.
Alternate Names
H2AFX
Gene Symbol
H2AX
Additional Histone H2AX Products
Product Documents for Histone H2AX Antibody (RM214)
Product Specific Notices for Histone H2AX Antibody (RM214)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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