Histone Deacetylase 8/HDAC8 Antibody (1O2Y5)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16713
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, ELISA, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 1O2Y5 expressed in HEK293
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Histone Deacetylase 8/HDAC8 (Q9BY41). MEEPEEPADSGQSLVPVYIYSPEYVSMCDSLAKIPKRASMVHSLIEAYALHKQMRIVKPKVASMEEMATFHTDAYLQHLQKVSQEGDDDHPDSIEYGLGY
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
42 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
Novus Biologicals Knockout (KO) Validated Rabbit Histone Deacetylase 8/HDAC8 Antibody (1O2Y5) (NBP3-16713) is a recombinant monoclonal antibody validated for use in IHC, WB, ICC/IF and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for Histone Deacetylase 8/HDAC8 Antibody (1O2Y5)
![Immunohistochemistry-Paraffin: Histone Deacetylase 8/HDAC8 Antibody (1O2Y5) [NBP3-16713]](https://resources.bio-techne.com/images/products/Histone-Deacetylase-8-HDAC8-Antibody-1O2Y5-Immunohistochemistry-Paraffin-NBP3-16713-img0002.jpg "Immunohistochemistry-Paraffin: Histone Deacetylase 8/HDAC8 Antibody (1O2Y5) [NBP3-16713]")
Immunohistochemistry-Paraffin: Histone Deacetylase 8/HDAC8 Antibody (1O2Y5) [NBP3-16713]
Immunohistochemistry-Paraffin: Histone Deacetylase 8/HDAC8 Antibody (1O2Y5) [NBP3-16713] - Rat brain using Histone Deacetylase 8/HDAC8 Rabbit mAb (NBP3-16713) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.![Immunoprecipitation: Histone Deacetylase 8/HDAC8 Antibody (1O2Y5) [NBP3-16713]](https://resources.bio-techne.com/images/products/Histone-Deacetylase-8-HDAC8-Antibody-1O2Y5-Immunoprecipitation-NBP3-16713-img0008.jpg "Immunoprecipitation: Histone Deacetylase 8/HDAC8 Antibody (1O2Y5) [NBP3-16713]")
Immunoprecipitation: Histone Deacetylase 8/HDAC8 Antibody (1O2Y5) [NBP3-16713]
Immunoprecipitation: Histone Deacetylase 8/HDAC8 Antibody (1O2Y5) [NBP3-16713] - Analysis of 300ug extracts of HeLa cells using 3ug Histone Deacetylase 8/HDAC8 antibody (NBP3-16713). Western blot was performed from the immunoprecipitate using Histone Deacetylase 8/HDAC8 antibody (NBP3-16713) at a dilition of 1:1000.
Immunocytochemistry/ Immunofluorescence: Histone Deacetylase 8/HDAC8 Antibody (1O2Y5) [NBP3-16713] -
Immunocytochemistry/ Immunofluorescence: Histone Deacetylase 8/HDAC8 Antibody (1O2Y5) [NBP3-16713] - Confocal imaging of HeLa cells using [KO Validated] Histone Deacetylase 8/HDAC8 Rabbit mAb. The cells were counterstained with alpha-Tubulin Mouse mAb (Green). DAPI was used for nuclear staining (blue). Objective: 100x.Applications for Histone Deacetylase 8/HDAC8 Antibody (1O2Y5)
Application
Recommended Usage
ELISA
Recommended starting concentration is 1 μg/mL.
Immunocytochemistry/ Immunofluorescence
1:50 - 1:200
Immunohistochemistry
1:100 - 1:1000
Immunohistochemistry-Paraffin
1:100 - 1:1000
Immunoprecipitation
0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.3), 50% glycerol, 0.05% BSA
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: Histone Deacetylase 8/HDAC8
Alternate Names
HDAC8, HDACL1, RPD3
Gene Symbol
HDAC8
Additional Histone Deacetylase 8/HDAC8 Products
Product Documents for Histone Deacetylase 8/HDAC8 Antibody (1O2Y5)
Product Specific Notices for Histone Deacetylase 8/HDAC8 Antibody (1O2Y5)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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