HIST1H2AB Antibody (1H12) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15228

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-15228
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Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 1H12 expressed in HEK293

Format

BSA Free

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all HIST1H2AB Antibodies »

Product Specifications

Immunogen

A synthesized peptide. UniProt ID (P04908).

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Description

Novus Biologicals Rabbit HIST1H2AB Antibody (1H12) - BSA Free (NBP3-15228) is a recombinant monoclonal antibody validated for use in WB, ELISA and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for HIST1H2AB Antibody (1H12) - BSA Free

Western Blot: HIST1H2AB Antibody (1H12) [NBP3-15228]

Western Blot: HIST1H2AB Antibody (1H12) [NBP3-15228]

Western Blot: HIST1H2AB Antibody (1H12) [NBP3-15228] - Positive WB detected in HepG2 whole cell lysate. All lanes: HIST1H2AB antibody at 2.7ug/ml. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 13 KDa. Observed band size: 13 Kda.
Immunocytochemistry/ Immunofluorescence: HIST1H2AB Antibody (1H12) [NBP3-15228]

Immunocytochemistry/ Immunofluorescence: HIST1H2AB Antibody (1H12) [NBP3-15228]

Immunocytochemistry/Immunofluorescence: HIST1H2AB Antibody (1H12) [NBP3-15228] - Immunofluorescence staining of Hela cells with at HIST1H2AB antibody 1:168, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C.The secondary antibody was Alexa Fluor 488-congugated Goat Anti-Rabbit IgG (H+L).

Applications for HIST1H2AB Antibody (1H12) - BSA Free

Application
Recommended Usage

Western Blot

1:500-1:5000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, pH 7.4, 150mM NaCl, and 50% glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -70C. Avoid freeze-thaw cycles.

Background: HIST1H2AB

HIST1H2AB is 130 amino acids long, weighs approximately 14 kDa, and is part of a group of basic nuclear proteins called histones, which are responsible for nucleosome structure of the chromosomal fiber in eukaryotes. Current studies are being done on several diseases and disorders including riddle syndrome, systemic lupus erythematosus, lupus erythematosus, cockayne syndrome, hemochromatosis, and immunodeficiency. HIST1H2AB has also been shown to have interactions with HIST1H4A, HIST1H4B, HIST1H4C, HIST1H4D, and HIST1H4E in pathways such as the proteasome degradation, telomere maintenance, nucleosome assembly, cell cycle, and systemic lupus erythematosus pathways.

Alternate Names

H2A/m, H2AFM, histone cluster 1, H2ab, histone H2A type 1-B/E

Gene Symbol

H2AC4

Additional HIST1H2AB Products

Product Documents for HIST1H2AB Antibody (1H12) - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for HIST1H2AB Antibody (1H12) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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