His Tag Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-25939
![Western Blot: His Tag AntibodyBSA Free [NBP1-25939]](https://resources.bio-techne.com/images/products/His-Tag-Antibody-Western-Blot-NBP1-25939-img0001.jpg "Western Blot: His Tag AntibodyBSA Free [NBP1-25939]")
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Conjugate
Catalog #
Key Product Details
Species Reactivity
Validated:
Epitope Tag
Cited:
Rat
Applications
Validated:
Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation
Cited:
Western Blot, Immunocytochemistry/ Immunofluorescence, IF/IHC
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Format
BSA Free
Concentration
1.0 mg/ml
Product Specifications
Immunogen
This His Tag Antibody was developed against Keyhole limpet haemocyanin (KLH) conjugated H-H-H-H-H-H.
Specificity
This antibody recognizes the motif H-H-H-H-H-H and is therefore of value in detecting proteins containing histidine tags. This antibody may not detect all N-terminal (NT) histidine tagged proteins.
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for His Tag Antibody - BSA Free
Western Blot: His Tag AntibodyBSA Free [NBP1-25939]
Western Blot: His Tag Antibody [NBP1-25939] - 200, 100, or 50 ng of E. coli whole cell lysate expressing a multi-tag fusion protein. Antibody used for WB at 0.2 ug/ml (1:5,000). Detection: Chemiluminescence withan exposure time of 3 minutes.Applications for His Tag Antibody - BSA Free
Application
Recommended Usage
ELISA
1:1000 - 1:30000
Immunocytochemistry/ Immunofluorescence
1:100 - 1:400
Immunoprecipitation
1 - 4 ug/mg lysate
Western Blot
1:1000 - 1:5000
Application Notes
For coating plates in ELISA use antibody at 1:100 - 1:500.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS
Format
BSA Free
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: His Tag
References
1. Malhotra, A. (2009). Tagging for protein expression. Methods in Enzymology, Guide to Protein Purification, 2nd Edition, 463, 239-258. https://doi.org/10.1016/s0076-6879(09)63016-0
2. Terpe, K. (2003). Overview of tag protein fusions: from molecular and biochemical fundamentals to commercial systems. Applied Microbiology and Biotechnology, 60(5), 523-533. https://doi.org/10.1007/s00253-002-1158-6
3. Booth, W. T., Schlachter, C. R., Pote, S., Ussin, N., Mank, N. J., Klapper, V., ... Chruszcz, M. (2018). Impact of an N-terminal polyhistidine tag on protein thermal stability. ACS Omega, 3(1), 760-768. https://doi.org/10.1021/acsomega.7b01598
Long Name
Histidine Tag
Alternate Names
polyHistidine
Additional His Tag Products
Product Documents for His Tag Antibody - BSA Free
Product Specific Notices for His Tag Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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