Novus Biologicals Mouse His Tag Antibody (AD1.1.10R) - BSA Free (NBP3-07011) is a recombinant monoclonal antibody validated for use in WB, ELISA, Flow, ICC/IF and IP. Anti-His Tag Antibody: Cited in 8 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.

Applications for His Tag Antibody (AD1.1.10R) - BSA Free

Application

Recommended Usage

Flow Cytometry

0.025 ug/mL

Western Blot

0.2 ug/mL

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: His Tag

A his-tag (also known as histidine tag or polyhistidine tag) is a common epitope tag that typically consists of at least 6 histidine residues fused to either the carboxyl (C-) or amino (N-) terminus of a targeted recombinant protein to facilitate its purification and detection (1). The most common his-tag is the hexahistidine (His6/6-His) tag which has a theoretical molecular weight of 0.8kda (1). The histidine residues readily interact with transition metal ions such as Co2+, Ni2+, Cu2+, and Zn2+, making immobilized metal-affinity chromatography (IMAC) the preferred technique for his-tag purification (1, 2). Metal ions are immobilized and bound to by His-tags in the IMAC column via the histidine imidazole ring. The tagged protein can be eluted off the column by washing with buffers containing a low concentration of imidazole (1, 2). Due to its relatively small size, low immunogenicity, versatility under denaturing conditions, and minimal interference with the structure and function of proteins, the his-tag is one of the most widely used tags for protein purification (1-3).

References

1. Malhotra, A. (2009). Tagging for protein expression. Methods in Enzymology, Guide to Protein Purification, 2nd Edition, 463, 239-258. https://doi.org/10.1016/s0076-6879(09)63016-0

2. Terpe, K. (2003). Overview of tag protein fusions: from molecular and biochemical fundamentals to commercial systems. Applied Microbiology and Biotechnology, 60(5), 523-533. https://doi.org/10.1007/s00253-002-1158-6

3. Booth, W. T., Schlachter, C. R., Pote, S., Ussin, N., Mank, N. J., Klapper, V., ... Chruszcz, M. (2018). Impact of an N-terminal polyhistidine tag on protein thermal stability. ACS Omega, 3(1), 760-768. https://doi.org/10.1021/acsomega.7b01598

Long Name

Histidine Tag

Alternate Names

polyHistidine

Additional His Tag Products

Product Documents for His Tag Antibody (AD1.1.10R) - BSA Free

Product Datasheets

Download Product Datasheets

COA

SDS

Download SDS

Product Specific Notices for His Tag Antibody (AD1.1.10R) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Novus Biologicals, part of Bio-Techne | Catalog # NBP3-07011

Key Product Details

Species Reactivity

Validated:

Cited:

Applications

Validated:

Cited:

Label

Antibody Source

Format

Concentration

View all His Tag Antibodies »

Product Specifications

Immunogen

Clonality

Host

Isotype

Description

Applications for His Tag Antibody (AD1.1.10R) - BSA Free

Flow Cytometry

Western Blot

Formulation, Preparation, and Storage

Purification

Formulation

Format

Preservative

Concentration

Stability & Storage

Background: His Tag

Long Name

Alternate Names

Additional His Tag Products

Product Documents for His Tag Antibody (AD1.1.10R) - BSA Free

Product Datasheets

COA

Certificate of Analysis

SDS

Product Specific Notices for His Tag Antibody (AD1.1.10R) - BSA Free