Hexokinase 1 Antibody (23GB870)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-52008

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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Knockdown Validated

Label

Unconjugated

Antibody Source

Monoclonal Rabbit IgG Clone # 23GB870

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Hexokinase 1 Antibodies »

Product Specifications

Immunogen

A synthesized peptide derived from human Hexokinase 1 (Uniprot: P19367)

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Hexokinase 1 Antibody (23GB870)

Hexokinase 1 Antibody (23GB870)

Immunocytochemistry/ Immunofluorescence: Hexokinase 1 Antibody (23GB870)[NBP3-52008] -

Immunocytochemical staining of HT-1080 cells with Hexokinase 1 antibody (1:1,000). Nuclei were stained blue with DAPI; Hexokinase 1 was stained magenta with Alexa Fluor(R) 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 um.
Hexokinase 1 Antibody (23GB870)

Flow Cytometry: Hexokinase 1 Antibody (23GB870)[NBP3-52008] -

Validation of Hexokinase 1 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with Hexokinase 1 antibody (1:2,000) and analyzed using CytoFLEX.
Hexokinase 1 Antibody (23GB870)

Flow Cytometry: Hexokinase 1 Antibody (23GB870)[NBP3-52008] -

Flow cytometric analysis of Hexokinase 1expression in HT-1080 cells using Hexokinase 1 antibody (isotype control; red, Hexokinase 1

Applications for Hexokinase 1 Antibody (23GB870)

Application
Recommended Usage

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Knockdown Validated

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.4), 50% glycerol

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Hexokinase 1

Hexokinases phosphorylate glucose to produce glucose-6-phosphate, thus committing glucose to the glycolytic pathway. This gene encodes a ubiquitous form of hexokinase which localizes to the outer membrane of mitochondria. Mutations in this gene have been associated with hemolytic anemia due to hexokinase deficiency. Alternative splicing of this gene results in five transcript variants which encode different isoforms, some of which are tissue-specific. Each isoform has a distinct N-terminus; the remainder of the protein is identical among all the isoforms. A sixth transcript variant has been described, but due to the presence of several stop codons, it is not thought to encode a protein.

Alternate Names

HK1, HKI

Gene Symbol

HK1

Additional Hexokinase 1 Products

Product Documents for Hexokinase 1 Antibody (23GB870)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Hexokinase 1 Antibody (23GB870)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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