HES5 Antibody (4S4U7)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16880
Recombinant Monoclonal Antibody
Key Product Details
Species Reactivity
Mouse, Rat
Applications
ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 4S4U7 expressed in HEK293
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 12-142 of human HES5 (Q5TA89). SPKEKNRLRKPVVEKMRRDRINSSIEQLKLLLEQEFARHQPNSKLEKADILEMAVSYLKHSKAFVAAAGPKSLHQDYSEGYSWCLQEAVQFLTLHAASDTQMKLLYHFQRPPAAPAAPAKEPKAPGAAPPP
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
18 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Description
Novus Biologicals Rabbit HES5 Antibody (4S4U7) (NBP3-16880) is a recombinant monoclonal antibody validated for use in IHC, WB and ELISA. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for HES5 Antibody (4S4U7)
![Western Blot: HES5 Antibody (4S4U7) [NBP3-16880]](https://resources.bio-techne.com/images/products/HES5-Antibody-4S4U7-Western-Blot-NBP3-16880-img0003.jpg "Western Blot: HES5 Antibody (4S4U7) [NBP3-16880]")
Western Blot: HES5 Antibody (4S4U7) [NBP3-16880]
Western Blot: HES5 Antibody (4S4U7) [NBP3-16880] - Analysis of extracts of various cell lines, using HES5 Rabbit mAb (NBP3-16880) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
Immunohistochemistry: HES5 Antibody (4S4U7) [NBP3-16880] -
Immunohistochemistry: HES5 Antibody (4S4U7) [NBP3-16880] - Immunohistochemistry analysis of paraffin-embedded Rat testis tissue using HES5 Rabbit mAb at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.Applications for HES5 Antibody (4S4U7)
Application
Recommended Usage
ELISA
Recommended starting concentration is 1 μg/mL
Immunohistochemistry
1:100-1:500
Immunohistochemistry-Paraffin
1:100-1:500
Western Blot
1:1000 - 1:6000
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS (pH 7.3), 50% glycerol, 0.05% BSA
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: HES-5
Long Name
Hairy And Enhancer of Split 5
Alternate Names
HES5
Gene Symbol
HES5
Additional HES-5 Products
Product Documents for HES5 Antibody (4S4U7)
Product Specific Notices for HES5 Antibody (4S4U7)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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