Hemoglobin Antibody (2A1) - Chimeric - Azide and BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-20120
Recombinant Monoclonal Antibody
Conjugate
Catalog #
Key Product Details
Species Reactivity
Mouse
Applications
Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Kappa Clone # 2A1
Format
Azide and BSA Free
Concentration
1 mg/ml
Product Specifications
Immunogen
Murine hybridomas were generated using spleen cells from aging (8-10 month old) NZB/W F1 animals. Hybridomas secreting antibodies reactive to murine hemoglobin were subcloned by limiting dilution and antibody isotypes determined using commercial reagents. Eight-week-old NZB/W F1 were immunized subcutaneously with 50 mg mHb antigen emulsified in Complete Fruend's Adjuvant.
Specificity
2A1 exhibited polyreactive recognition of peptide 100-119 from the alpha subunit and peptide 100-119 from the beta subunit. Moreover, 2A1 bound Hb and the Hb-haptoglobin complex with equal efficiency, implying different binding sites on Hb for haptoglobin and 2A1. 2A1 was also found to distinguish between Hb and metHb.
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG Kappa
Description
Novus Biologicals Rabbit Hemoglobin Antibody (2A1) - Chimeric - Azide and BSA Free (NBP3-20120) is a recombinant monoclonal antibody validated for use in WB, ELISA and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
Scientific Data Images for Hemoglobin Antibody (2A1) - Chimeric - Azide and BSA Free
Immunocytochemistry/Immunofluorescence: Hemoglobin Antibody (2A1) - Chimeric - Azide and BSA Free [NBP3-20120] -
Immunocytochemistry/Immunofluorescence: Hemoglobin Antibody (2A1) - Chimeric - Azide and BSA Free [NBP3-20120] - Immunofluorescence analysis of paraformaldehyde fixed mouse splenocytes on Shi-fix(TM) coverslips, permeabilized with 0.15% Triton stained with the chimeric rabbit IgG version of 2A1 (NBP3-20120) at 10 ug/ml for 1h followed by Alexa Fluor(R) 488 secondary antibody (2 ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP3-20120, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody followed by staining with Alexa Fluor(R) 488 secondary antibody.Applications for Hemoglobin Antibody (2A1) - Chimeric - Azide and BSA Free
Application
Recommended Usage
ELISA
Optimal dilutions of this antibody should be experimentally determined.
Immunocytochemistry/ Immunofluorescence
Optimal dilutions of this antibody should be experimentally determined.
Western Blot
Optimal dilutions of this antibody should be experimentally determined.
Application Notes
This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG format, for improved compatibility with existing reagents, assays and techniques.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS
Format
Azide and BSA Free
Preservative
0.02% Proclin 300
Concentration
1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C for up to 3 months. For longer storage, aliquot and store at -20C.
Background: Hemoglobin
Hemoglobin is a tetrameric protein that serves as the oxygen transporter in red blood cells. Two alpha chain and two beta chains comprise the adult form of the molecule, with each subunit containing a heme group, or oxygen binding site. Sickle cell anemia is caused by a single amino acid change in the beta chain, which alters the solubility of the deoxygenated form of the molecule and causes the formation of long fibers that distort the red blood cell into the characteristic sickle shape. Thalassemias, another set of genetic diseases associated with hemoglobin, result from absence or deficiency in one of the hemoglobin chains.
Alternate Names
alpha one globin;alpha-1 globin;alpha-1-globin;alpha-2 globin chain;Alpha-globin;ALPHA-GLOBIN GENE;delta globin;Haemoglobin;Hb;HbA1c;hemoglobin alpha 1 globin chain;Hemoglobin alpha chain;Hemoglobin subunit alpha;hemoglobin, alpha 1
Additional Hemoglobin Products
Product Documents for Hemoglobin Antibody (2A1) - Chimeric - Azide and BSA Free
Product Specific Notices for Hemoglobin Antibody (2A1) - Chimeric - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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