Helicobacter Pylori Antibody (MSVA-466M)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-57263
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2b Kappa Clone # MSVA-466M
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Recombinant Helicobacter pylori Catalase protein fragment (around aa323-445) (Exact sequence is proprietary) Swissprot Not Applicable
Localization
Cell surface. Cytoplasm.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2b Kappa
Description
This antibody is highly specific and optimized for use in Immunohistochemistry.
It has been validated on TMAs covering 76 normal tissues and numerous cancer tissues.
The antibody concentration is optimized for dilution within dilution range using commercially available antibody diluent for immunohistochemistry.
Positive Control
Postive control:Gastric tissue with known helicobacter pylori infection should show a distinct staining of bacteria on the surface and/or within glands..
Negative Control
Negitve control:Gastric tissue: Normal epithelial and stromal cells should not stain.
Storage
Antibody can be stored at 4degC. For extended storage, aliquot and store at -20degC. Avoid repeated freeze-thaw cycles.
Applications for Helicobacter Pylori Antibody (MSVA-466M)
Application
Recommended Usage
Immunohistochemistry-Paraffin
1:100 1:200
Application Notes
Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.
Formulation, Preparation, and Storage
Purification
Protein A or G purified
Formulation
10mM PBS
Preservative
0.1% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C.
Background: Helicobacter Pylori
Alternate Names
H. pylori
Additional Helicobacter Pylori Products
Product Documents for Helicobacter Pylori Antibody (MSVA-466M)
Product Specific Notices for Helicobacter Pylori Antibody (MSVA-466M)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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