HDAC1 Antibody (CL0510) - Azide and BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-44032

Novus Biologicals, part of Bio-Techne
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NBP3-44032
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BSA Free
NBP2-52937
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Key Product Details

Validated by

Knockout/Knockdown, Orthogonal Validation

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # CL0510

Format

Azide and BSA Free

Concentration

LYOPH mg/ml

View all Histone Deacetylase 1/HDAC1 Antibodies »

Product Specifications

Immunogen

This antibody was generated using a recombinant protein sequence of Q13547, with the exact immunogen sequence remaining proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Description

Novus Biologicals Mouse HDAC1 Antibody (CL0510) - Azide and BSA Free (NBP2-52937) is a monoclonal antibody validated for use in IHC, WB and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for HDAC1 Antibody (CL0510) - Azide and BSA Free

HDAC1 Antibody (CL0510) - Azide and BSA Free Immunohistochemistry-Paraffin: HDAC1 Antibody [NBP3-44032]

Immunohistochemistry-Paraffin: HDAC1 Antibody [NBP3-44032]

Analysis in human rectum and skeletal muscle tissues using NBP3-44032 antibody. Corresponding HDAC1 RNA-seq data are presented for the same tissues.
HDAC1 Antibody (CL0510) - Azide and BSA Free Immunohistochemistry-Paraffin: HDAC1 Antibody [NBP3-44032]

Immunohistochemistry-Paraffin: HDAC1 Antibody [NBP3-44032]

Staining of human fallopian tube shows strong nuclear positivity in glandular cells.
HDAC1 Antibody (CL0510) - Azide and BSA Free Immunohistochemistry-Paraffin: HDAC1 Antibody [NBP3-44032]

Immunohistochemistry-Paraffin: HDAC1 Antibody [NBP3-44032]

Staining of human skeletal muscle shows no positivity in myocytes as expected.
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Applications for HDAC1 Antibody (CL0510) - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

2-10 ug/ml

Immunohistochemistry-Paraffin

1:1000 - 1:2500

Western Blot

1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. For Immunocytochemistry/ Immunofluorescence/IF, PFA/Triton X-100 is recommended for fixation/permeabilization.

Formulation, Preparation, and Storage

Purification

Protein A purified

Reconstitution

Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Formulation

Lyophilized from a 0.2 um filtered solution in PBS with Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Histone Deacetylase 1/HDAC1

Histone acetylation and deacetylation, catalyzed by multisubunit complexes, play a key role in the regulation of eukaryotic gene expression. The protein encoded by this gene belongs to the histone deacetylase/acuc/apha family and is a component of the histone deacetylase complex. It also interacts with retinoblastoma tumor-suppressor protein and this complex is a key element in the control of cell proliferation and differentiation. Together with metastasis-associated protein-2, it deacetylates p53 and modulates its effect on cell growth and apoptosis.

Long Name

Histone Deacetylase 1

Alternate Names

GON-10, HD1, KDAC1, RPD3L1

Gene Symbol

HDAC1

Additional Histone Deacetylase 1/HDAC1 Products

Product Documents for HDAC1 Antibody (CL0510) - Azide and BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for HDAC1 Antibody (CL0510) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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