H2A [ac Lys5] Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-48612

Novus Biologicals, part of Bio-Techne
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NBP3-48612
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Dot Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all H2A Antibodies »

Product Specifications

Immunogen

Carrier-protein conjugated synthetic peptide surrounding acetyl Lys5 of human H2A. The exact sequence is proprietary.

Modification

ac Lys5

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

Novus Biologicals Rabbit H2A [ac Lys5] Antibody (NBP3-48612) is a polyclonal antibody validated for use in WB, ICC/IF and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for H2A [ac Lys5] Antibody

H2A [ac Lys5] Antibody

Dot Blot: H2A [ac Lys5] Antibody [NBP3-48612] -

Dotblot analysis of anti-H2A antibody with peptide samples.
Varied amount of peptide samples were spotted onto positively charged nylon membrane and blotted with H2A antibody (NBP3-48612) 1:5000 dilution.
A: Peptide samples of H2A.2K5 un
B: Peptide samples of H2A.2K5ac
C: Peptide samples of H2BK20 un
D: Peptide samples of H2BK20ac
E: Peptide samples of H2BK5 un
F: Peptide samples of H2BK5ac
H2A [ac Lys5] Antibody

Western Blot: H2A [ac Lys5] Antibody [NBP3-48612] -

H2A antibody detects H2A protein by Western blot analysis.
A. 30 ug HeLa whole cell lysate/extract (0.012% DMSO treatment for 18 hr)
B. 30 ug HeLa whole cell lysate/extract (0.4 uM Trichostatin A treatment for 18 hr)
15 % SDS-PAGE
H2A antibody (NBP3-48612) dilution: 1:10000
H2A [ac Lys5] Antibody

Immunoprecipitation: H2A [ac Lys5] Antibody [NBP3-48612] -

H2A antibody immunoprecipitates H2A protein in IP experiments. IP Sample: HeLa whole cell lysate/extract A : 30 ug whole cell lysate/extract of H2A protein expressing HeLa cells (0.4uM TSA treatment for 18hr) B : Control with 2.5 ug of pre-immune rabbit IgG C : Immunoprecipitation of H2A protein by 2.5 ug of H2A antibody (NBP3-48612) 12% SDS-PAGE The immunoprecipitated H2A protein was detected by H2A antibody (NBP3-48612) diluted at 1 : 10000. EasyBlot anti-rabbit IgG (HRP) was used as a secondary reagent.

Applications for H2A [ac Lys5] Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunoprecipitation

1:100-1:10000

Western Blot

1:1000-1:20000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: H2A

H2A is 130 amino acids long, weighs approximately 14 kDa, and is part of a group of basic nuclear proteins called histones, which are responsible for nucleosome structure of the chromosomal fiber in eukaryotes. Current studies are being done on several diseases and disorders including systemic lupus erythematosus, lupus erythematosus, x inactivation, riddle syndrome, connective tissue disease, and immunodeficiency. H2A has also been shown to have interactions with BMI1, DNAJC2, RCC1, RNF2, and SRRM1 in pathways such as the telomere maintenance, nucleosome assembly, cell cycle, and systemic lupus erythematosus pathways.

Alternate Names

H2A histone family, member O, H2A.2, H2A/O, H2A/q, H2a-615, H2AFOHistone H2A/o, HIST2H2AAH2A, histone 2, H2aa, histone 2, H2aa3, histone cluster 2, H2aa3, histone H2A type 2-A, Histone H2A.2

Gene Symbol

HIST2H2AA3

Additional H2A Products

Product Documents for H2A [ac Lys5] Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for H2A [ac Lys5] Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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